Description

5α-Androstane-3β,17β-Diol-[D4] (Dihydroepiandrosterone-[D4]) (Solution) is a high-purity, deuterium-labeled internal standard critical for advanced analytical quantification. This stable isotope-labeled compound is essential for ensuring the accuracy and reliability of mass spectrometry-based assays by correcting for analyte loss during sample preparation and instrument variability. It is primarily utilized by pharmaceutical R&D laboratories, clinical research organizations, and forensic toxicology labs for the precise measurement of endogenous steroid hormones and their metabolites in complex biological matrices.

Application

• Internal Standard for LC-MS/MS: Used as a quantitative reference in liquid chromatography-tandem mass spectrometry for analyzing DHEA and related steroid pathways in plasma, serum, and urine.
• Endocrinology Research: Enables precise study of androgen metabolism, adrenal function, and hormonal disorders in clinical and preclinical research settings.
• Doping Control Analysis: Supports anti-doping agencies in the sensitive and confirmatory detection of prohibited anabolic androgenic steroids in athlete samples.
• Pharmaceutical Bioanalysis: Facilitates pharmacokinetic and pharmacodynamic studies for drug candidates targeting hormonal systems, ensuring compliant data for regulatory submissions.
• Metabolomics & Biomarker Discovery: Serves as a critical tool in steroid profiling for identifying disease biomarkers in oncology, endocrinology, and metabolic syndrome research.

Basic Information

Product Name	5α-Androstane-3β,17β-Diol-[D4] (Dihydroepiandrosterone-[D4]) (Solution)
CAS No.	5352-59-0
Molecular Formula	C19H26D4O2
Molecular Weight	294.47 g/mol
Synonyms	5α-Androstane-3β,17β-diol-16,16,17,17-d4; DHEA-d4; Androstanediol-d4; 3β,17β-Dihydroxy-5α-androstane-d4; Deuterated Dihydroepiandrosterone; [16,16,17,17-2H4]-5α-Androstane-3β,17β-diol; 5α-Androstanediol-d4; Isotopic Internal Standard DHEA-d4
EINECS	Contact for details

Quality Control

Every batch of our deuterated internal standards is produced and verified under strict quality management to ensure isotopic purity and chemical integrity. The synthesis and purification processes are designed to meet the exacting requirements of GMP/GLP-compliant laboratories. Comprehensive analytical documentation, including detailed Certificates of Analysis (COA) with lot-specific data from NMR, HPLC, and MS, is provided to support your method validation and regulatory compliance.

Storage

Preserve in the original tightly closed container, protected from light. Store at the recommended temperature as specified on the certificate of analysis, typically between 2°C and 8°C for long-term stability. The solution is prepared in a compatible organic solvent; handle with appropriate precautions for strong acids/organic solvents in a well-ventilated area.

Specification

Item	Specification
Appearance	Clear, colorless to pale yellow solution
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Chemical Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (MS)	≥ 98 atom % D
Concentration	1.0 mg/mL (or as specified)
Solvent	Methanol, Acetonitrile, or other specified

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.