Description

4-Aminophenyl-β-D-Galactopyranoside is a specialized glycoside derivative that serves as a critical chromogenic and fluorogenic substrate for enzyme detection. This compound matters for its high specificity in biochemical assays, enabling precise measurement of β-galactosidase activity. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies developing cell-based reporter systems and diagnostic tools.

Application

• Biochemical Research: As a key substrate for detecting and quantifying β-galactosidase enzyme activity in molecular biology and genetics research.
• Diagnostic Assays: Used in the development of clinical and laboratory diagnostic kits for bacterial detection (e.g., E. coli) and genetic disorders.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms within drug discovery to identify modulators of gene expression using reporter gene systems (e.g., lacZ).
• Cell Biology & Histochemistry: Utilized in X-gal staining for visualizing β-galactosidase expression in tissues, embryos, and cultured cells.
• Enzyme Characterization: Serves as a standard substrate for kinetic studies and inhibitor testing of β-galactosidase enzymes.
• Food & Water Safety Testing: Applied in microbiological methods for the detection of coliform bacteria as an indicator of contamination.

Basic Information

Product Name	4-Aminophenyl-β-D-Galactopyranoside
CAS No.	5094-33-7
Molecular Formula	C12H17NO6
Molecular Weight	271.27 g/mol
Synonyms	4-Aminophenyl β-D-galactopyranoside; P-Aminophenyl β-D-galactopyranoside; APG; 4-APG; p-Aminophenyl-β-D-galactoside; 4-Aminophenyl-β-D-galactoside; β-Galactosidase substrate (APG); 4-APGal
EINECS	225-806-9

Quality Control

Our 4-Aminophenyl-β-D-Galactopyranoside is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is kept sealed in a desiccated environment to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm
Solubility	Clear, colorless solution in water or DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.