Description

Naphthol As-Bi Phosphate Disodium Salt is a high-purity biochemical reagent widely used as a chromogenic substrate in enzymatic histochemistry and cytochemistry. This compound is critical for the precise detection and localization of alkaline phosphatase activity in tissue sections, a fundamental technique in diagnostic pathology and life science research. It is an essential component for laboratories and manufacturers in the fields of in-vitro diagnostics (IVD), biomedical research, and histology reagent production.

Application

• Histochemistry & Cytochemistry: Primary substrate for the detection of alkaline phosphatase (ALP) activity in tissue sections and cell smears.
• In-Vitro Diagnostics (IVD): Key reagent in the formulation of kits for enzyme-linked immunoassays and other diagnostic platforms.
• Biomedical Research: Used in studies involving cell differentiation, signal transduction, and biomarker discovery.
• Pathology & Clinical Diagnostics: Enables visualization of enzyme activity in biopsy samples for disease diagnosis and research.
• Reagent Manufacturing: Sourced by chemical suppliers for the production of high-grade staining kits and laboratory reagents.

Basic Information

Product Name	Naphthol As-Bi Phosphate Disodium Salt
CAS No.	530-79-0
Molecular Formula	C23H15NNa2O6P
Molecular Weight	493.32 g/mol
Synonyms	Naphthol AS-BI phosphate disodium salt; 3-Hydroxy-4-nitrophenyl 2-naphthoate phosphate disodium salt; Naphthol AS-BI phosphoric acid disodium salt; AS-BI phosphate; Phosphoric acid 2-naphthol-3-carboxanilide 6-ester disodium salt; Fast Blue RR salt substrate; Alkaline phosphatase substrate; Naphthol AS-BI-PO4 Na2
EINECS	208-500-9

Quality Control

Our Naphthol As-Bi Phosphate Disodium Salt is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR), to ensure it meets the stringent requirements for diagnostic and research applications. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to guarantee traceability and compliance.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a low-humidity environment to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 5.0%
Solubility	Clear, yellow solution in water
Heavy Metals (as Pb)	≤ 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.