Description

Puromycin Dihydrochloride is a potent aminonucleoside antibiotic widely used as a critical selection agent in molecular biology and cell culture research. Its primary value lies in its ability to rapidly and irreversibly inhibit protein synthesis, making it an indispensable tool for stable cell line selection and the study of gene function. This high-purity compound is essential for researchers and manufacturers in the biotechnology, pharmaceutical development, and life sciences sectors who require reliable and consistent performance in their experimental and production workflows.

Application

• Stable Cell Line Selection: Used as a selective agent in mammalian cell culture for the establishment of stable cell lines expressing puromycin N-acetyltransferase (PAC) resistance genes.
• Gene Knockdown Validation: Employed to select for cells successfully transduced with lentiviral or retroviral vectors, commonly used in CRISPR-Cas9, shRNA, and other gene editing/knockdown studies.
• Antibiotic Mode of Action Research: Serves as a model compound in biochemical studies investigating the mechanisms of protein synthesis inhibition and ribosomal function.
• Biopharmaceutical Production: Utilized in the development and manufacturing of therapeutic proteins, vaccines, and viral vectors using engineered cell lines that confer puromycin resistance.
• Microbiology Studies: Acts as an effective antibiotic against prokaryotic and eukaryotic cells in various microbiological assays.

Basic Information

Product Name	Puromycin Dihydrochloride
CAS No.	58-58-2
Molecular Formula	C₂₂H₂₉N₇O₅ • 2HCl
Molecular Weight	544.43 g/mol
Synonyms	Puromycin 2HCl; CL 16536; NSC 3055; Stylomycin; Achromycin; 6-Dimethylamino-9-[3'-deoxy-3'-(p-methoxy-L-phenylalanylamino)-β-D-ribofuranosyl]purine dihydrochloride; 3'-[α-Amino-p-methoxyhydrocinnamamido]-3'-deoxy-N,N-dimethyladenosine dihydrochloride
EINECS	200-387-6

Quality Control

Our Puromycin Dihydrochloride is manufactured under strict quality management systems to ensure batch-to-batch consistency and high biological activity. Each lot undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and MS, and rigorous sterility and endotoxin testing where applicable for cell culture use. Certificates of Analysis (COA) detailing all specifications and test results are provided to guarantee compliance with your research and production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and potency.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Assay (HPLC)	≥98.0%
Water Content (KF)	≤5.0%
pH (1% Solution)	3.0 - 5.0
Biological Activity (Cell Culture)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.