Description

Aminopterin is a potent folic acid antagonist and classical antimetabolite with significant biochemical activity. This compound is a critical research tool for studying cellular metabolism, enzyme inhibition, and the mechanisms of antifolate drugs. It is primarily utilized by researchers and scientists in the fields of biochemistry, oncology research, and cell biology for experimental studies.

Application

• Biochemical Research: Used as a selective inhibitor of dihydrofolate reductase (DHFR) to study folate metabolism and nucleotide biosynthesis pathways.
• Oncology & Cancer Research: Serves as a reference compound and tool for investigating the mechanisms of action of antifolate chemotherapeutic agents.
• Cell Culture & Selection: Employed in laboratory settings for the selection of recombinant cells in genetic engineering and microbiology studies.
• Enzyme Inhibition Studies: Acts as a potent inhibitor in enzymatic assays to characterize DHFR activity and screen for potential inhibitors.
• Pharmacological Research: Used in preclinical studies to model the effects of folate antagonism and understand related toxicities.

Basic Information

Product Name	Aminopterin
CAS No.	54-62-6
Molecular Formula	C19H20N8O5
Molecular Weight	440.41 g/mol
Synonyms	4-Aminofolic acid; 4-Aminopteroylglutamic acid; NSC 739; 4-Amino-PGA; L-Glutamic acid, N-[4-[[(2,4-diamino-6-pteridinyl)methyl]amino]benzoyl]-; Aminopterine; 2,4-Diamino-4-deoxypteroylglutamate; 4-Aminopteroyl-L-glutamate
EINECS	200-209-9

Quality Control

Our Aminopterin is produced and handled under strict quality management protocols to ensure high purity and batch-to-batch consistency for research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles. We adhere to relevant standards for research chemicals to guarantee reliable and reproducible performance in your laboratory.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term stability, consider storage under inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.