Description

Puromycin is a potent aminonucleoside antibiotic that functions as a protein synthesis inhibitor by causing premature chain termination during translation. This specific mechanism of action makes it an indispensable tool in molecular and cell biology research for selecting genetically modified cells. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical R&D departments engaged in genetic engineering and stable cell line development.

Application

• Stable Cell Line Selection: The most common application, used as a selective agent in mammalian cell culture to isolate cells expressing puromycin N-acetyl-transferase (PAC) resistance genes.
• Gene Expression Studies: Employed in research involving lentiviral and retroviral transduction to select for successfully transduced cells, enabling the study of gene function and regulation.
• Antibiotic Mode of Action Research: Serves as a model compound for studying ribosomal function, translation inhibition, and mechanisms of antibiotic resistance.
• Apoptosis Induction: Used in experimental protocols to induce cell death in control populations or to study cellular stress responses.
• Microbiological Research: Although primarily a research tool, its antibiotic properties are relevant in studies targeting certain protozoa and microbes.

Basic Information

Product Name	Puromycin
CAS No.	53-79-2
Molecular Formula	C22H29N7O5
Molecular Weight	471.52 g/mol
Synonyms	Puromycin Dihydrochloride (common salt form); CL 16536; NSC 3055; Stylomycin; Achromycin; 3'-Deoxy-N,N-dimethyl-3'-oxoadenosine; 6-Dimethylamino-9-[3-deoxy-3-(p-methoxy-L-phenylalanylamino)-β-D-ribofuranosyl]purine
EINECS	200-185-8

Quality Control

Our Puromycin is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for critical research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, potency, and sterility where applicable. We adhere to high-purity standards suitable for cell culture and molecular biology use. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥98.0%
Water Content (KF)	≤5.0%
pH (1% Solution)	5.0 - 7.0
Biological Activity	Tested for effective selection concentration
Endotoxin (LAL)	<1.0 EU/mg (for cell culture grade)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.