Description

m-PEG3-NHS Carbonate is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a carbonate group for hydroxyl modification. This compound is essential for creating stable, hydrophilic spacers in bioconjugation, significantly improving the solubility and pharmacokinetic profiles of therapeutic molecules. It is primarily utilized by researchers and manufacturers in the pharmaceutical and biotech industries for developing advanced antibody-drug conjugates (ADCs), peptide therapeutics, and diagnostic reagents.

Application

• Conjugation of amines (e.g., lysine residues on proteins/antibodies) to hydroxyl-containing molecules (e.g., drugs, fluorophores).
• Critical reagent in the synthesis of antibody-drug conjugates (ADCs) and other targeted therapeutics.
• Introduction of hydrophilic PEG spacers to reduce immunogenicity and improve solubility of bioconjugates.
• Surface functionalization of nanoparticles and biomaterials for drug delivery systems.
• Modification of peptides and oligonucleotides to enhance stability and bioavailability.
• Preparation of enzyme-polymer conjugates for industrial biocatalysis.
• Development of diagnostic assays and biosensors through controlled immobilization strategies.

Basic Information

Product Name	m-PEG3-NHS Carbonate
CAS No.	477775-77-2
Molecular Formula	C15H23NO9
Molecular Weight	361.35 g/mol
Synonyms	m-PEG3-NHS Carbonate; 1-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-1-oxo-2,5,8,11-tetraoxatridecan-13-yl carbonochloridate; NHS-PEG3-carbonate; Carbonic acid 2,5-dioxo-1-pyrrolidinyl 2,5,8,11-tetraoxatridecan-13-yl ester; 13-[[(2,5-Dioxo-1-pyrrolidinyl)oxy]carbonyl]-2,5,8,11-tetraoxatridecanoic acid 2,5-dioxo-1-pyrrolidinyl ester; mPEG3-NHS carbonate ester; Heterobifunctional PEG crosslinker NHS-PEG3-carbonate
EINECS	Contact for details

Quality Control

Our m-PEG3-NHS Carbonate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for critical bioconjugation applications. Certificates of Analysis (COA) with detailed specifications are provided to guarantee compliance with your research and cGMP manufacturing requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This material is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize hydrolysis of the active NHS ester.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.