Description

C12 Nbd L-Threo-Sphingosine CAS NO 474943-08-3 is a fluorescently labeled derivative of the essential sphingolipid backbone, L-threo-sphingosine, featuring a C12 fatty acyl chain and the nitrobenzoxadiazole (NBD) fluorophore. This compound is a critical research tool for the real-time visualization and tracking of sphingolipid metabolism, membrane dynamics, and intracellular trafficking in live-cell systems. It is primarily utilized by researchers and scientists in the fields of lipid biochemistry, cell biology, and pharmaceutical development to study signaling pathways and develop novel therapeutic agents.

Application

• Live-Cell Imaging and Fluorescence Microscopy: As a fluorescent probe for real-time visualization of sphingolipid localization and movement within cellular membranes.
• Sphingolipid Metabolism Studies: Tracing the metabolic fate and enzymatic processing of sphingosine derivatives in biological systems.
• Membrane Biophysics Research: Investigating lipid raft formation, membrane fluidity, and protein-lipid interactions using fluorescence-based techniques.
• Intracellular Trafficking Assays: Monitoring the transport and sorting of lipids through secretory and endocytic pathways.
• Drug Discovery & Target Validation: Serving as a key reagent in high-content screening assays to identify modulators of sphingolipid-related enzymes or pathways.
• Academic and Industrial R&D: Fundamental research tool in universities, research institutes, and biotech/pharma companies focused on cancer, neurology, and metabolic diseases.

Basic Information

Product Name	C12 Nbd L-Threo-Sphingosine
CAS No.	474943-08-3
Molecular Formula	C30H48N4O5
Molecular Weight	544.73 g/mol
Synonyms	N-[(1E,3S)-3-Hydroxy-1-(hydroxymethyl)-5-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-1-pentadecen-1-yl]dodecanamide; NBD C12-Sphingosine; N-(12-((7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)dodecanoyl)-L-threo-sphingosine; D-erythro-Sphingosine, N-(12-((7-nitro-2,1,3-benzoxadiazol-4-yl)amino)dodecanoyl)-, (2S,3R)-; Fluorescent C12 Sphingosine; L-threo-C12-NBD-Ceramide precursor
EINECS	Contact for details

Quality Control

Our C12 Nbd L-Threo-Sphingosine is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive biological research. Each lot is characterized by HPLC, NMR, and mass spectrometry to confirm chemical identity and purity. Certificates of Analysis (COA) detailing all analytical results are provided with every shipment. We adhere to ISO 9001 quality management standards in our production and control processes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize condensation and moisture uptake. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Solubility	Soluble in DMSO, ethanol, DMF
Fluorescence	λex ~465 nm, λem ~535 nm (in methanol)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.