Description

n-[12-[(7-Nitro-2-1,3-Benzoxadiazol-4-Yl)Amino]Dodecanoyl]-Sphinganine is a specialized fluorescently labeled sphingolipid derivative, designed for advanced research and diagnostic applications. This compound is critical for the study of lipid metabolism, trafficking, and cellular signaling pathways due to its high-affinity labeling properties. It is primarily utilized by researchers and developers in the fields of biochemistry, cell biology, and pharmaceutical development, where precise molecular tracking is essential.

Application

• Biochemical Research: As a fluorescent probe for studying sphingolipid metabolism, membrane dynamics, and intracellular transport mechanisms.
• Cell Biology Studies: For labeling and visualizing lipid rafts, endocytic pathways, and organelle-specific lipid distribution in live or fixed cells.
• Diagnostic Assay Development: Serving as a key reagent in the development of high-sensitivity fluorescence-based assays for enzyme activity (e.g., sphingomyelinases, ceramidases).
• Pharmaceutical Screening: Used in target-based screening platforms to identify and characterize modulators of sphingolipid-related enzymes and receptors.
• Lipidomics: As an internal standard or reference compound in mass spectrometry-based lipidomic analyses for accurate quantification.
• Neuroscience Research: Investigating the role of sphingolipids in neuronal function, myelination, and neurodegenerative disease models.

Basic Information

Product Name	n-[12-[(7-Nitro-2-1,3-Benzoxadiazol-4-Yl)Amino]Dodecanoyl]-Sphinganine
CAS No.	474943-05-0
Molecular Formula	C₃₆H₆₁N₅O₆
Molecular Weight	659.91 g/mol
Synonyms	NBD C12-Ceramide; NBD C12-D-erythro-Sphinganine; 7-Nitrobenz-2-oxa-1,3-diazol-4-yl (NBD) C12 Ceramide; N-[12-[(7-Nitro-4-benzofurazanyl)amino]dodecanoyl]-D-erythro-sphinganine; N-(12-((7-Nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)dodecanoyl)sphinganine; Fluorescent Ceramide C12-NBD; NBD C12 Sphinganine; NBD-labeled C12 Ceramide
EINECS	Contact for details

Quality Control

Our n-[12-[(7-Nitro-2-1,3-Benzoxadiazol-4-Yl)Amino]Dodecanoyl]-Sphinganine is produced under strict quality management systems to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. A detailed Certificate of Analysis (COA) documenting identity, purity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to light, moisture, or elevated temperatures should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange solid or powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 1.0 %
Solubility	Soluble in DMSO, ethanol, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.