Description

Phosphoramidous Acid. N.n-Bis(1-Methylethyl)-. 2-Cyanoethyl [1-(2-Nitrophenyl)Ethyl] Ester) CAS NO 461425-49-0 is a specialized phosphoramidite derivative, a crucial building block in modern synthetic chemistry. Its primary value lies in its role as a key monomer for the automated synthesis of oligonucleotides, enabling the production of DNA and RNA sequences with high precision and yield. This compound is essential for researchers and manufacturers in the fields of pharmaceutical development, molecular diagnostics, and biotechnology, where reliable, high-purity chemical intermediates are non-negotiable.

Application

• Oligonucleotide Synthesis: Serves as a critical phosphoramidite monomer in solid-phase automated DNA/RNA synthesizers.
• Antisense Therapy Development: Used in the research and production of antisense oligonucleotides for therapeutic applications.
• PCR Primer and Probe Manufacturing: Essential for creating high-fidelity primers and labeled probes used in genetic testing and diagnostics.
• siRNA and miRNA Synthesis: A key raw material for synthesizing small interfering RNA and microRNA for gene silencing studies.
• Next-Generation Sequencing (NGS): Employed in the fabrication of oligonucleotide libraries and capture probes.
• Academic and Industrial R&D: Used in chemical biology research for nucleic acid modification and probe design.

Basic Information

Product Name	Phosphoramidous Acid. N.n-Bis(1-Methylethyl)-. 2-Cyanoethyl [1-(2-Nitrophenyl)Ethyl] Ester)
CAS No.	461425-49-0
Molecular Formula	C19H30N3O4P
Molecular Weight	395.44 g/mol
Synonyms	1-(2-Nitrophenyl)ethyl diisopropylphosphoramidite; 2-Cyanoethyl N,N-diisopropylphosphoramidite of 1-(2-nitrophenyl)ethanol; [1-(2-Nitrophenyl)ethyl] 2-cyanoethyl N,N-diisopropylphosphoramidite; Diisopropyl-[1-(2-nitrophenyl)ethoxy]-(2-cyanoethoxy)phosphine; Phosphoramidite monomer for photolabile protecting groups; Photocleavable phosphoramidite; 2-Nitrobenzyl-derived phosphoramidite.
EINECS	Contact for details

Quality Control

Our production of this high-value phosphoramidite adheres to stringent quality protocols to ensure batch-to-batch consistency and optimal performance in sensitive synthesis applications. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and 31P NMR to confirm identity, purity, and the absence of critical impurities like hydrolyzed species. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at temperatures between -20°C to -10°C under an inert atmosphere (argon or nitrogen) due to its moisture-sensitive and light-sensitive nature. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Colorless to pale yellow clear liquid or solid
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 97.0%
Water Content (Karl Fischer)	≤ 0.5%
Solubility	Freely soluble in anhydrous acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.