Description

1,17-Bisbiotinylamino-3,6,9,12,15-Pentaoxaheptadecane is a specialized heterobifunctional crosslinking reagent featuring two biotin moieties connected by a hydrophilic polyethylene glycol (PEG) spacer. This compound is engineered to enable high-affinity capture and detection of biomolecules through the strong biotin-streptavidin interaction, while the PEG linker enhances solubility and reduces non-specific binding. It is an essential tool for researchers and developers in the fields of bioconjugation, diagnostic assay development, and affinity chromatography, where precise and stable labeling is required.

Application

• Bioconjugation and Protein Labeling: For creating biotinylated antibodies, enzymes, and other proteins for use in immunoassays (ELISA, Western Blot).
• Diagnostic Kit Development: As a critical component in lateral flow assays and other point-of-care diagnostic devices for signal amplification.
• Affinity Purification Systems: Immobilization onto solid supports (e.g., agarose beads) for the capture and purification of streptavidin/avidin-tagged molecules.
• Cell Surface Labeling and Imaging: For tagging cell membrane proteins for visualization via fluorescence microscopy using streptavidin-fluorophore conjugates.
• Drug Delivery and Targeting: In the development of biotinylated nanoparticles or liposomes for targeted therapeutic delivery.
• Biosensor Fabrication: Functionalization of sensor surfaces to create highly specific detection platforms for biomarkers.
• Molecular Biology: Labeling of nucleic acids (DNA, RNA) for pull-down assays and microarray applications.

Basic Information

Product Name	1,17-Bisbiotinylamino-3,6,9,12,15-Pentaoxaheptadecane
CAS No.	440680-87-5
Molecular Formula	C38H68N8O12S2
Molecular Weight	893.13 g/mol
Synonyms	Bis-Biotin-PEG5; Biotin-PEG5-Biotin; 1,17-Bis(biotinylamino)-3,6,9,12,15-pentaoxaheptadecane; PEG5-Bis-Biotin; Biotin-(PEG)5-Biotin; Heterobifunctional PEG-Biotin Crosslinker; Biotinylated PEG Spacer; EZ-Link™ Bis-Biotin-PEG5 (analog)
EINECS	Contact for details

Quality Control

Our 1,17-Bisbiotinylamino-3,6,9,12,15-Pentaoxaheptadecane is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and structural confirmation via mass spectrometry (MS) and NMR. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all specification results. Our quality commitment ensures the reproducibility and performance required for sensitive life science research and in-vitro diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. Under these conditions, the product is stable for at least 24 months. Allow the vial to warm to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms (M+H+ or M+Na+)
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.