Description

5-Bromo-4-Chloro-3-Indoxyl Choline Phosphate is a specialized chromogenic substrate used in enzymatic detection and diagnostic assays. This compound is critical for the sensitive and specific visualization of enzymatic activity, particularly for phosphatases, due to its ability to produce an insoluble, colored precipitate upon cleavage. It is an essential reagent for researchers and manufacturers in the life sciences, diagnostics, and pharmaceutical development sectors seeking reliable, high-purity biochemical tools.

Application

• Histochemistry & Cytochemistry: As a substrate for the detection and localization of alkaline phosphatase and other phosphatase enzyme activity in tissue sections and cell cultures.
• Diagnostic Assay Development: A key component in the formulation of chromogenic kits for clinical diagnostics, including ELISA and immunoblotting applications.
• Microbiology & Bacteriology: Used in culture media and identification systems to detect specific bacterial enzymes, aiding in microbial characterization.
• Biochemical Research: Employed in enzyme kinetics studies and high-throughput screening (HTS) assays to monitor phosphatase activity.
• Pharmaceutical R&D: Utilized in drug discovery platforms to identify and characterize inhibitors or activators of phosphatase targets.
• In-situ Hybridization: Serves as a detection reagent in nucleic acid hybridization techniques when coupled with enzyme-labeled probes.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indoxyl Choline Phosphate
CAS No.	439809-43-5
Molecular Formula	C13H17BrClN2O6P
Molecular Weight	443.61 g/mol
Synonyms	BCIP-Choline Phosphate; 5-Bromo-4-chloro-3-indolyl phosphorylcholine; 3-Indolyl phosphate, 5-bromo-4-chloro-, choline salt; Choline 5-Bromo-4-chloro-3-indolyl phosphate; BCIP-ChP; Phosphorylcholine 5-Bromo-4-chloro-3-indoxyl ester; (5-Bromo-4-chloro-1H-indol-3-yl) dihydrogen phosphate choline salt
EINECS	Contact for details

Quality Control

Every batch of 5-Bromo-4-Chloro-3-Indoxyl Choline Phosphate is manufactured and tested under strict quality management systems. We provide comprehensive analytical data to ensure the product meets the high-purity standards required for sensitive biochemical applications. A Certificate of Analysis (COA) detailing purity, identity, and performance characteristics is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO
Heavy Metals	< 10 ppm
Performance Test (Enzymatic)	Pass

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.