Description

Methotrexate-Methyl-D3, Dimethyl Ester is a stable isotope-labeled derivative of the antifolate drug methotrexate, specifically deuterated on the methyl group of the dimethyl ester. This compound is a critical reference standard and internal standard for the precise quantification and metabolic study of methotrexate in complex biological matrices. It is essential for researchers and quality control laboratories in the pharmaceutical and biotechnology sectors who require high-fidelity analytical tools for method development, validation, and pharmacokinetic research.

Application

• Pharmaceutical Reference Standard: Serves as a certified primary standard for calibrating analytical instruments and validating assay methods for methotrexate in drug substance and product testing.
• Mass Spectrometry Internal Standard: Used in Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) and GC-MS for the accurate, isotope-dilution quantification of methotrexate in pharmacokinetic and bioequivalence studies.
• Drug Metabolism and Pharmacokinetics (DMPK) Research: Enables detailed investigation of the absorption, distribution, metabolism, and excretion (ADME) profile of methotrexate.
• Clinical Research and Therapeutic Drug Monitoring (TDM): Facilitates the development of highly sensitive and specific assays to monitor methotrexate levels in patient serum or plasma, optimizing dosing regimens and minimizing toxicity.
• Impurity Profiling and Degradation Studies: Acts as a critical tool in identifying and quantifying potential deuterated impurities or degradation products in methotrexate formulations.
• Biomedical Research: Supports fundamental research into the mechanisms of action, resistance, and folate metabolism in oncology and autoimmune disease models.

Basic Information

Product Name	Methotrexate-Methyl-D3, Dimethyl Ester
CAS No.	432545-60-3
Molecular Formula	C₂₂H₂₀D₃N₈O₅
Molecular Weight	478.48 g/mol
Synonyms	Methotrexate-d3 (dimethyl ester); L-Glutamic acid, N-[4-[[(2,4-diamino-6-pteridinyl)methyl]methyl-d3-amino]benzoyl]-, dimethyl ester; (2S)-2-[[4-[(2,4-Diaminopteridin-6-yl)methyl-methyl-d3-amino]benzoyl]amino]pentanedioic acid dimethyl ester; Deuterated Methotrexate Dimethyl Ester; MTX-d3 Dimethyl Ester; Methotrexate Methyl-d3 Ester
EINECS	Contact for details

Quality Control

Every batch of Methotrexate-Methyl-D3, Dimethyl Ester is manufactured and analyzed under strict quality management systems. We guarantee high chemical and isotopic purity, with comprehensive characterization using advanced techniques including NMR (¹H, ¹³C), HPLC, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, isotopic enrichment, and other critical specifications to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.