Description

6-Chloro-3-Indoxyl-β-D-Cellobioside is a specialized chromogenic substrate used for the detection of specific enzymatic activity. This compound is critical for research and diagnostic applications where precise and sensitive visualization of biological processes is required. It is primarily utilized by scientists and quality control professionals in the biotechnology, pharmaceutical, and life science research sectors.

Application

• Enzyme Substrate for β-Glucosidase/Cellulase: Acts as a specific chromogenic substrate for the detection and assay of β-glucosidase and cellulase enzyme activity.
• Microbiological Research: Used in microbial identification and characterization studies, particularly for organisms expressing relevant hydrolytic enzymes.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits and reagents for clinical and research laboratories.
• Biomass Conversion Studies: Employed in research focused on cellulose degradation and biofuel production processes to monitor enzymatic breakdown.
• Histochemistry & Cell Biology: Used in staining protocols to localize enzyme activity within tissues and cell cultures.
• High-Throughput Screening (HTS): Suitable for developing assays in drug discovery platforms targeting glycosidase enzymes.
• Food & Agricultural Science: Applied in quality control and research related to enzyme activity in food processing and agricultural products.
• Industrial Enzyme Monitoring: Used to quantify and monitor the activity of commercial enzyme preparations in various industrial processes.

Basic Information

Product Name	6-Chloro-3-Indoxyl-β-D-Cellobioside
CAS No.	425427-87-8
Molecular Formula	C20H26ClNO11
Molecular Weight	491.87 g/mol
Synonyms	6-Chloro-3-indolyl-β-D-cellobioside; 6-Cl-3-Indoxyl-β-D-Cellobioside; 6-Chloro-3-indoxyl β-cellobioside; 6-Chloroindoxyl-β-D-cellobioside; 5-Chloro-3-indolyl β-D-cellobioside (common numbering variant); Chromogenic β-glucosidase substrate; Cellobioside indoxyl derivative; BCIP/AS-MX substrate analog (related); X-Cellobioside (trade name style).
EINECS	Contact for details

Quality Control

Our 6-Chloro-3-Indoxyl-β-D-Cellobioside is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is analyzed via HPLC, NMR, and mass spectrometry to confirm identity and purity. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). This product is light-sensitive and should be handled under appropriate conditions to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.