Description

Pro-Arg 4-Methoxy-β-Naphthylamide Acetate Salt is a specialized biochemical reagent, a derivative of the chromogenic substrate 4-methoxy-2-naphthylamine, designed for high-sensitivity enzymatic assays. Its core value lies in its role as a substrate for specific proteases, where enzymatic cleavage releases a detectable, colored product, enabling precise quantification of enzyme activity. This compound is essential for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science sectors requiring reliable and sensitive detection methods for proteolytic enzymes.

Application

• Protease Activity Assays: Primary substrate for the detection and quantification of trypsin, thrombin, and other arginine-specific proteases in research and diagnostic kits.
• Diagnostic Reagent Manufacturing: A key component in the formulation of chromogenic substrates for clinical chemistry analyzers and point-of-care testing devices.
• Biochemical Research: Used in enzyme kinetics studies, inhibitor screening, and characterization of protease function in cellular pathways.
• Pharmaceutical Quality Control: Employed in potency testing and stability studies of biopharmaceuticals that are proteases or contain protease activity.
• Histochemistry & Cell Biology: Can be utilized in situ for localizing protease activity in tissue sections or cell cultures.

Basic Information

Product Name	Pro-Arg 4-Methoxy-β-Naphthylamide Acetate Salt
CAS No.	42761-75-1
Molecular Formula	C₂₄H₃₄N₆O₆ • xC₂H₄O₂
Molecular Weight	502.57 g/mol (free base)
Synonyms	L-Prolyl-L-arginine 4-methoxy-2-naphthylamide acetate salt; Pro-Arg-4-MeO-βNA Acetate; BOC-Pro-Arg-4-Methoxy-β-Naphthylamide Acetate (common precursor); Chromogenic substrate for trypsin; Tosyl-Gly-Pro-Arg-4-methoxy-2-naphthylamide acetate (related derivative); Protease substrate 42761-75-1; L-Pro-L-Arg-4-MeO-βNA • AcOH
EINECS	Contact for details

Quality Control

Our Pro-Arg 4-Methoxy-β-Naphthylamide Acetate Salt is produced under strict quality management protocols to ensure batch-to-batch consistency and reliability for critical assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms to reference standard in trypsin assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.