Description

Dispase I is a highly purified, neutral protease enzyme derived from *Bacillus polymyxa*, widely recognized for its gentle and specific action in dissociating tissues without damaging cell membranes. Its primary value lies in its ability to cleave fibronectin and collagen IV, making it indispensable for the isolation of intact, viable cells from a variety of epithelial and other tissues. This product is essential for researchers and manufacturers in cell culture, tissue engineering, and primary cell isolation applications, where maintaining high cell viability and function is critical.

Application

• Primary Cell Isolation: Gentle dissociation of epithelial cells, hepatocytes, keratinocytes, and endothelial cells from tissues.
• Stem Cell Research: Harvesting of embryonic stem cells and other pluripotent stem cell colonies with minimal damage.
• Tissue Engineering & Regenerative Medicine: Preparation of cell suspensions for 3D bioprinting and scaffold seeding.
• Cancer Research: Isolation of tumor cells from biopsy specimens for in vitro studies and drug screening.
• Organoid Culture: Dissociation of tissues to initiate and maintain complex 3D organoid models.
• Cell Subculture: Detachment of adherent cell lines, particularly those sensitive to trypsin.

Basic Information

Product Name	Dispase I
CAS No.	42613-33-2
Molecular Formula	N/A (Enzyme Preparation)
Molecular Weight	~35-40 kDa (Protein component)
Synonyms	Neutral Protease Dispase; Bacillus polymyxa Neutral Protease; Dispase Neutral Protease; EC 3.4.24.4; Microbial Neutral Metalloprotease; Tissue Dissociation Enzyme; Fibronectinase; Collagenase Type IV; Dispase II (related enzyme)
EINECS	Contact for details

Quality Control

Our Dispase I is manufactured and tested under a quality management system to ensure batch-to-batch consistency and performance. Each lot is characterized for specific activity, purity, and the absence of contaminating proteases. A comprehensive Certificate of Analysis (COA) detailing assay, sterility (where applicable), endotoxin levels, and performance in cell dissociation is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive). For long-term stability, desiccate at -20°C. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to light brown lyophilized powder
Identification (Activity)	Conforms (Specific Cell Dissociation)
Assay (Specific Activity)	≥ 4.0 units/mg protein
Purity (HPLC)	≥ 90% (Protein basis)
Endotoxin	< 10 EU/mg (for cell culture grade)
Solubility	Soluble in water or balanced salt solutions
Loss on Drying	< 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.