Description

Nitrocefin CAS NO 41906-86-9 is a chromogenic cephalosporin substrate specifically designed for the detection of β-lactamase activity. This compound is a critical diagnostic reagent, undergoing a distinct color change from yellow to red upon hydrolysis by β-lactamase enzymes. It is an essential tool for microbiology laboratories, pharmaceutical quality control, and antibiotic research. The product is primarily used by clinical diagnostics manufacturers, pharmaceutical R&D departments, and academic research institutions focused on antimicrobial resistance.

Application

• β-Lactamase Detection: Primary substrate in colorimetric and spectrophotometric assays for identifying β-lactamase-producing bacteria.
• Clinical Diagnostics: Key component in commercial test kits and strips (e.g., Nitrocefin disks) for rapid phenotypic confirmation of ESBL and other resistant pathogens.
• Pharmaceutical Research: Used in high-throughput screening (HTS) to evaluate the efficacy of novel β-lactam antibiotics and β-lactamase inhibitors.
• Microbiology & AMR Studies: Essential for studying the kinetics, mechanism, and spectrum of β-lactamase enzymes in antimicrobial resistance (AMR) surveillance.
• Quality Control in Antibiotic Manufacturing: Employed to test for β-lactamase contamination or to verify the stability of β-lactam drug products.
• Educational & Training Kits: Used in university and hospital training programs to demonstrate the principles of bacterial antibiotic resistance.

Basic Information

Item	Details
Product Name	Nitrocefin
CAS No.	41906-86-9
Molecular Formula	C₂₁H₁₈N₆O₈S₂
Molecular Weight	546.53 g/mol
Synonyms	(6R,7R)-7-[(2Z)-2-(2-Amino-1,3-thiazol-4-yl)-2-[[(2S)-3,3-dimethyl-4-oxo-4,5-dihydro-1,3-thiazol-2-ylidene]methoxyimino]acetamido]-8-oxo-3-[(E)-1-(2-nitrophenyl)diazen-1-yl]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid; Nitrocefin sodium salt; Cefinase; Chromogenic cephalosporin; Glaxo 87/312; β-lactamase substrate; BD Nitrocefin; Oxoid Nitrocefin
EINECS	Contact for details

Quality Control

Our Nitrocefin is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, spectral analysis for identity confirmation, and microbiological performance testing to ensure consistent and reliable activity as a β-lactamase substrate. Certificates of Analysis (COA) detailing purity, assay, and performance specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, consider storage under desiccant or inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Assay (Enzymatic Activity)	≥ 90% activity vs. reference standard
Solubility	Soluble in DMSO, sparingly soluble in water
Extinction Coefficient	Confirmed within specified range

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.