Description

Ac-Ala-Ala-Ala-Pna CAS NO 40817-33-2 is a synthetic tripeptide derivative, specifically an acetylated alanine sequence coupled to a p-nitroanilide (Pna) chromogenic group. This compound is a critical substrate for the study and detection of proteolytic enzyme activity, particularly serine and cysteine proteases. It is essential for researchers and manufacturers in the fields of biochemical research, pharmaceutical development, and diagnostic assay kits where precise enzyme kinetics and inhibition studies are required.

Application

• Protease Activity Assays: Primary chromogenic substrate for quantifying the activity of enzymes like elastase, chymotrypsin, and subtilisin.
• Enzyme Kinetic Studies: Used in research to determine kinetic parameters (Km, Vmax) and inhibition constants (Ki) for various proteases.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify and characterize novel protease inhibitors.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for conditions related to abnormal protease levels.
• Biochemical Research Tool: Fundamental reagent for studying protease structure, function, and specificity in academic and industrial laboratories.
• Quality Control in Biologics: Used to monitor and validate the absence of specific protease activities in biopharmaceutical production processes.

Basic Information

Product Name	Ac-Ala-Ala-Ala-Pna
CAS No.	40817-33-2
Molecular Formula	C20H28N6O7
Molecular Weight	464.47 g/mol
Synonyms	N-Acetyl-L-alanyl-L-alanyl-L-alanine 4-nitroanilide; Ac-AAA-pNA; Ac-Ala-Ala-Ala-pNA; Acetyl-Ala-Ala-Ala-p-nitroanilide; N-Acetyl-Ala-Ala-Ala-pNA; Suc-(Ala)3-pNA; Protease Substrate III (Elastase Substrate); Elastase Chromogenic Substrate
EINECS	Contact for details

Quality Control

Our Ac-Ala-Ala-Ala-Pna is produced under stringent quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, mass spectrometry for identity confirmation, and spectrophotometric assays for functional activity. We provide Certificates of Analysis (COA) detailing all specifications to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.