Description

2-Methylcoelenterazine is a synthetic, cell-permeable luminogenic substrate for Renilla luciferase, widely used as a critical reagent in bioluminescence assays. Its modified structure offers enhanced stability and light output characteristics compared to native coelenterazine, making it a preferred choice for sensitive detection. This compound is essential for researchers and manufacturers in the fields of molecular biology, drug discovery, and cellular imaging who require reliable, high-performance tools for reporter gene assays, ATP detection, and calcium signaling studies.

Application

• Reporter Gene Assays: Primary substrate for Renilla luciferase in dual-luciferase reporter systems for gene expression analysis.
• Bioluminescence Resonance Energy Transfer (BRET): Used as the energy donor in BRET-based protein-protein interaction studies and cellular signaling pathway analysis.
• ATP Detection & Cell Viability Assays: Employed in ultrasensitive bioluminescent assays to quantify ATP levels as a marker of cellular metabolism and viability.
• Calcium Ion Sensing: Utilized in aequorin-based bioluminescent systems for real-time monitoring of intracellular calcium concentration changes.
• High-Throughput Screening (HTS): A key component in target-based and cell-based screening campaigns within pharmaceutical R&D.
• In Vivo Imaging: Applied in small animal imaging studies to track tumor growth, gene expression, and other biological processes non-invasively.

Basic Information

Product Name	2-Methylcoelenterazine
CAS No.	40040-83-3
Molecular Formula	C₂₆H₂₁N₃O₂
Molecular Weight	407.47 g/mol
Synonyms	2-Methylcoelenterazine; 2-Methyl-coelenterazine; 2-Methyl CTZ; Coelenterazine 2-methyl; 6-(4-Methoxyphenyl)-2-[(4-methoxyphenyl)methyl]-3,7-dihydroimidazo[1,2-a]pyrazin-8(2H)-one; 6-(p-Methoxyphenyl)-2-[(p-methoxyphenyl)methyl]imidazo[1,2-a]pyrazin-3(7H)-one; DeepBlueC (DBC) substrate; CLZN-2M
EINECS	Contact for details

Quality Control

Our 2-Methylcoelenterazine is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high performance in sensitive biological assays. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to support your research and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below under an inert atmosphere (e.g., argon or nitrogen). This product is strictly light-sensitive (protect from all light exposure), easily oxidized (store under inert atmosphere), and hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Bioluminescence Activity	Conforms to performance standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.