Description

L-Leucine-p-Nitroanilide is a specialized chromogenic substrate primarily used in enzymatic assays and biochemical research. This compound is critical for the study and quantification of protease and peptidase activity, particularly leucine aminopeptidase. It is an essential reagent for pharmaceutical R&D, diagnostic kit manufacturing, and academic research in enzymology and molecular biology. The product is supplied as a high-purity analytical standard to ensure reliable and reproducible experimental results.

Application

• Enzymatic Substrate for Aminopeptidases: Primary substrate for the colorimetric and spectrophotometric assay of leucine aminopeptidase (LAP) activity.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) to identify and characterize potential enzyme inhibitors for drug discovery.
• Clinical Diagnostics: Key component in the formulation of diagnostic kits for measuring LAP levels in biological samples, relevant for liver and pancreatic function tests.
• Biochemical Research: Essential tool for studying enzyme kinetics, specificity, and mechanism of action in academic and industrial laboratories.
• Quality Control in Biotechnology: Employed to assess the purity and activity of enzyme preparations in biomanufacturing processes.

Basic Information

Product Name	L-Leucine-p-Nitroanilide
CAS No.	4578-93-2
Molecular Formula	C12H17N3O3
Molecular Weight	251.28 g/mol
Synonyms	L-Leucine 4-nitroanilide; L-Leu-p-NA; 4-Nitroanilide of L-leucine; N-(4-Nitrophenyl)-L-leucinamide; L-Leucyl-4-nitroaniline; Leucine p-nitroanilide; Substrate for Leucine Aminopeptidase; LAP Substrate
EINECS	Contact for details

Quality Control

Our L-Leucine-p-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, spectrophotometric analysis, and identity confirmation (IR, NMR) to ensure it meets the stringent requirements for research and diagnostic applications. A Certificate of Analysis (COA) detailing batch-specific results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Melting Point	185 - 190 °C
Absorbance (Ethanol)	Conforms
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.