Description

4-Morpholineethanesulfonic Acid is a high-purity biochemical buffer essential for maintaining stable pH conditions in sensitive biological and chemical systems. Its zwitterionic nature and excellent buffering capacity in the physiological pH range make it a critical reagent for research and industrial processes requiring precise environmental control. This product is primarily utilized by the biotechnology, pharmaceutical, and diagnostic industries for applications such as cell culture media, enzyme assays, and chromatography.

Application

• Biochemical & Molecular Biology Research: As a key component in buffer systems for protein purification, electrophoresis, and nucleic acid analysis.
• Pharmaceutical Formulation: Used in the development and manufacturing of biologics and vaccines to stabilize pH in final formulations and intermediate process steps.
• Cell Culture & Tissue Engineering: An essential buffering agent in cell culture media to maintain physiological pH for mammalian cell growth.
• Diagnostic Reagents: Incorporated into buffers for clinical diagnostic kits and assays to ensure consistent and accurate performance.
• Chromatography: Employed as a mobile phase modifier or running buffer in various chromatographic techniques, including HPLC and FPLC.
• Enzyme Kinetics Studies: Provides a stable pH environment for studying enzyme activity, inhibition, and characterization.

Basic Information

Product Name	4-Morpholineethanesulfonic Acid
CAS No.	4432-31-9
Molecular Formula	C6H13NO4S
Molecular Weight	195.24 g/mol
Synonyms	MES; 2-(N-Morpholino)ethanesulfonic Acid; 4-Morpholineethanesulfonic Acid; MES Buffer; Morpholine-4-ethanesulfonic acid; 2-Morpholinoethanesulfonic acid; 2-(4-Morpholino)ethanesulfonic acid; 4-Morpholinepropanesulfonic acid (common misnomer)
EINECS	224-632-3

Quality Control

Our 4-Morpholineethanesulfonic Acid is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing to ensure it meets high-purity standards suitable for critical research and GMP applications. A Certificate of Analysis (COA) is provided with each shipment, detailing parameters such as assay, pH, absorbance, and heavy metal content. We can supply material compliant with various pharmacopeial standards upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive). Keep the container tightly sealed in a dry environment to prevent absorption of atmospheric moisture, which can affect product quality.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms
Assay (HPLC)	≥ 99.0%
pH (1% Solution)	2.5 - 4.0
UV Absorbance (1M, 260 nm)	≤ 0.05
UV Absorbance (1M, 280 nm)	≤ 0.05
Water (KF)	≤ 0.5%
Heavy Metals (as Pb)	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.