Description

p-Nitrophenyl β-D-Lactopyranoside is a specialized chromogenic substrate used in enzymatic assays. This compound matters for its critical role in the detection and quantification of β-galactosidase activity, a key enzyme in molecular biology and diagnostic research. It is primarily needed by biotechnology, pharmaceutical, and diagnostic kit manufacturing industries for research, quality control, and assay development.

Application

• Enzymatic Substrate for β-Galactosidase: Used as a chromogenic substrate in colorimetric assays to detect and measure β-galactosidase activity in research and diagnostic applications.
• Molecular Biology Research: Essential in reporter gene assays (e.g., lacZ gene expression) for studying gene regulation and promoter activity.
• Diagnostic Kit Manufacturing: A key component in the formulation of diagnostic kits for microbiology and clinical chemistry, used to identify specific bacterial enzymes.
• Biochemical Research: Employed in enzyme kinetics studies to determine kinetic parameters like Km and Vmax for β-galactosidase.
• Cell Biology & High-Throughput Screening (HTS): Utilized in cell-based assays and HTS platforms for drug discovery and functional genomics.
• Food & Environmental Testing: Applied in methods for detecting coliform bacteria and E. coli in water and food samples.

Basic Information

Product Name	p-Nitrophenyl β-D-Lactopyranoside
CAS No.	4419-94-7
Molecular Formula	C18H23NO12
Molecular Weight	445.38 g/mol
Synonyms	4-Nitrophenyl β-D-lactopyranoside; p-Nitrophenyl β-D-lactoside; PNPL; 4-Nitrophenyl lactoside; β-D-Galactopyranosyl-(1→4)-β-D-glucopyranoside 4-nitrophenyl ester; O-4-Nitrophenyl-β-D-lactoside; Lactose 4-nitrophenyl β-D-galactopyranoside; pNP-β-Lactoside
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl β-D-Lactopyranoside is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing to ensure high purity and performance consistency for sensitive enzymatic applications. Certificates of Analysis (COA) with detailed specifications for identity, purity, and related substances are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled in a low-humidity environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Heavy Metals	≤ 20 ppm
Residual Solvents	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.