Description

Disodium 4-Nitrophenylphosphate is a high-purity biochemical reagent widely used as a chromogenic substrate for enzyme assays. This compound is critical for research and diagnostic applications where precise measurement of alkaline phosphatase activity is required. It is an essential tool for laboratories in the pharmaceutical, biotechnology, and clinical diagnostics industries.

Application

• Primary substrate for alkaline phosphatase (ALP) assays in clinical diagnostics and research.
• Key component in ELISA (Enzyme-Linked Immunosorbent Assay) kits and other immunoassay systems.
• Biochemical research reagent for studying enzyme kinetics and inhibition.
• Used in molecular biology for nucleic acid detection methods involving alkaline phosphatase-conjugated probes.
• Quality control testing in pharmaceutical manufacturing for enzyme activity validation.
• In-vitro diagnostic (IVD) device manufacturing as a standardized reagent.

Basic Information

Product Name	Disodium 4-Nitrophenylphosphate
CAS No.	4264-83-9
Molecular Formula	C6H6NNa2O6P
Molecular Weight	263.07 g/mol
Synonyms	4-Nitrophenyl Phosphate Disodium Salt; PNPP; p-Nitrophenyl Phosphate Disodium Salt; Disodium p-Nitrophenyl Phosphate; Phosphoric Acid 4-Nitrophenyl Ester Disodium Salt; ALP Substrate; Alkaline Phosphatase Substrate; 4-NPP; Di-Sodium 4-Nitrophenyl Phosphate
EINECS	224-247-1

Quality Control

Our Disodium 4-Nitrophenylphosphate is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive diagnostic and research applications. Each lot is tested to meet stringent specifications for assay, absorbance, solubility, and identity. A comprehensive Certificate of Analysis (COA) detailing purity, performance in enzymatic assays, and absence of interfering contaminants is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). Keep the container tightly sealed in a desiccated environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Absorbance (405 nm)	Meets performance criteria
Solubility (Water)	Clear, colorless solution
Loss on Drying	≤ 2.0%
Enzymatic Activity	Suitable for ALP assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.