Description

5-Bromo-4-Chloro-3-Indolyl-n-Acetyl-β-D-Glucosaminide is a specialized chromogenic substrate used in enzymatic assays for the detection of N-acetyl-β-D-glucosaminidase (NAGase) activity. This compound is critical for researchers and diagnostic manufacturers requiring precise, colorimetric detection methods in biochemical and clinical settings. It is primarily utilized in life science research, diagnostic kit development, and microbiology for studying enzyme kinetics and pathogen identification.

Application

• Diagnostic Reagent Manufacturing: As a key chromogenic component in clinical diagnostic kits for detecting NAGase activity, an indicator for certain renal and hepatic disorders.
• Microbiological Research: For the identification and study of bacteria, fungi, and other microorganisms that express N-acetyl-β-D-glucosaminidase.
• Enzyme Activity Assays: In biochemical research to measure and characterize NAGase enzyme kinetics and inhibition studies in vitro.
• Histochemistry & Cell Biology: Used in staining procedures to localize NAGase activity in tissue sections and cell cultures.
• Pharmaceutical Development: As a tool compound in drug discovery screens targeting glycosidase enzymes.
• Food & Environmental Testing: Potential application in assays designed to detect microbial contamination or specific enzymatic activity in samples.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl-n-Acetyl-β-D-Glucosaminide
CAS No.	4264-82-8
Molecular Formula	C16H18BrClN2O7
Molecular Weight	465.68 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl N-acetyl-β-D-glucosaminide; X-N-Acetyl-β-D-glucosaminide (where X=5-Br-4-Cl-3-indolyl); NAG Chromogenic Substrate; BCIP-NAG; 5-Br-4-Cl-3-Indolyl-β-D-GlcNAc; N-Acetyl-β-D-glucosaminide, 5-bromo-4-chloro-3-indolyl; 4264-82-8; Chromogenic NAG substrate.
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl-n-Acetyl-β-D-Glucosaminide is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) documenting purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.