Description

3'-Hydroxypentobarbital is a key pharmaceutical intermediate and reference standard, primarily known as a major metabolite of the sedative-hypnotic drug pentobarbital. This compound is essential for analytical and research purposes, particularly in forensic toxicology, pharmacokinetic studies, and drug metabolism research. It is a critical material for laboratories and manufacturers involved in developing and validating analytical methods, producing certified reference materials, and conducting clinical studies.

Application

• Pharmaceutical Reference Standard: Used as a certified reference material (CRM) for the qualitative and quantitative analysis of pentobarbital and its metabolites in various matrices.
• Forensic Toxicology: Serves as a critical analytical standard for the detection and confirmation of pentobarbital use in post-mortem and human performance testing.
• Metabolite Research: Employed in in vitro and in vivo studies to investigate the metabolic pathways, pharmacokinetics, and pharmacodynamics of pentobarbital.
• Clinical Study Support: Utilized in bioanalytical method development and validation for clinical trials involving barbiturate compounds.
• Quality Control: Acts as an impurity standard or system suitability check in the quality control of pentobarbital active pharmaceutical ingredient (API) and finished dosage forms.
• Academic Research: Used in university and institutional research projects focusing on neuropharmacology, anesthesia, and drug disposition.

Basic Information

Product Name	3'-Hydroxypentobarbital
CAS No.	4241-40-1
Molecular Formula	C11H18N2O4
Molecular Weight	242.27 g/mol
Synonyms	5-Ethyl-5-(3-hydroxy-1-methylbutyl)barbituric Acid; 3'-Hydroxy-5-ethyl-5-(1-methylbutyl)barbituric Acid; 5-Ethyl-5-(1-methyl-3-hydroxybutyl)barbituric Acid; Pentobarbital 3'-Hydroxy Metabolite; 3'-OH-Pentobarbital; NSC 74121
EINECS	Contact for details

Quality Control

Our 3'-Hydroxypentobarbital is produced and handled under a strict quality management system. Each batch undergoes comprehensive analytical testing, including identification by spectroscopic methods (IR, NMR), purity assay by HPLC, and control of related substances and residual solvents. A detailed Certificate of Analysis (COA) is provided with each shipment, ensuring traceability and compliance with your research and quality standards.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term storage, consider storing at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single unknown impurity ≤ 0.5%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Complies with ICH Q3C guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.