Description

[[(2R,3S,4R,5R)-5-(6-Aminopurin-9-Yl)-3,4-Dihydroxyoxolan-2-Yl]Methoxy-Hydroxyphosphoryl] 5-[(6S)-2-Oxo-1,3,3A,4,6,6A-Hexahydrothieno[3,4-D]Imidazol-6-Yl]Pentanoate is a high-purity, chemically defined nucleotide-biotin conjugate, essential for advanced biochemical research and diagnostic applications. This compound serves as a critical building block and labeling reagent in molecular biology, enabling the study and detection of nucleic acid-protein interactions. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers for developing sensitive assays and probes.

Application

• Bioconjugation and Labeling: Acts as a key reagent for the enzymatic or chemical incorporation of biotin tags into oligonucleotides for detection systems.
• Molecular Biology Research: Used in the study of nucleic acid metabolism, enzyme mechanisms (e.g., polymerases, ligases), and nucleic acid-protein binding assays.
• Diagnostic Assay Development: Serves as a critical component in the manufacturing of ELISA, microarray, and in-situ hybridization kits that require high-sensitivity biotin-streptavidin detection.
• Drug Discovery Platforms: Employed in high-throughput screening (HTS) assays to identify compounds that interact with specific DNA or RNA sequences.
• Next-Generation Sequencing (NGS): Can be used in probe-based target enrichment or library preparation steps that utilize biotinylated capture sequences.
• Affinity Purification: Facilitates the pull-down and isolation of specific nucleic acid-binding proteins or complexes using streptavidin-coated beads.

Basic Information

Product Name	[[(2R,3S,4R,5R)-5-(6-Aminopurin-9-Yl)-3,4-Dihydroxyoxolan-2-Yl]Methoxy-Hydroxyphosphoryl] 5-[(6S)-2-Oxo-1,3,3A,4,6,6A-Hexahydrothieno[3,4-D]Imidazol-6-Yl]Pentanoate
CAS No.	4130-20-5
Molecular Formula	C24H32N10O10PS
Molecular Weight	675.62 g/mol
Synonyms	Biotin-11-dATP; Biotin-11-2'-deoxyadenosine-5'-triphosphate; Biotin-11-dATP, Trisodium Salt; 5'-[N-(6-Biotinoylaminohexanoyl)-3-aminoallyl]-dATP; Biotinylated dATP; dATP-Biotin; Biotin-11-2'-deoxyadenosine triphosphate; N6-(6-((6-((Biotinoyl)amino)hexanoyl)amino)hexyl)-2'-deoxyadenosine-5'-triphosphate
EINECS	Contact for details

Quality Control

Our [[(2R,3S,4R,5R)-5-(6-Aminopurin-9-Yl)-3,4-Dihydroxyoxolan-2-Yl]Methoxy-Hydroxyphosphoryl] 5-[(6S)-2-Oxo-1,3,3A,4,6,6A-Hexahydrothieno[3,4-D]Imidazol-6-Yl]Pentanoate is manufactured under strict quality systems suitable for research and diagnostic use. Each batch is subjected to rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to ensure identity, purity, and functional performance. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant and under an inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with molecular structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Functional Activity	Suitable for enzymatic incorporation (tested)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.