Description

3-Indoxyl-β-D-Glucopyranoside is a specialized chromogenic substrate used in enzymatic assays. This compound is critical for the detection and quantification of β-glucosidase activity, serving as a key reagent in diagnostic and research applications. It is primarily utilized by professionals in the biotechnology, pharmaceutical, and life science research sectors for applications ranging from enzyme kinetics studies to high-throughput screening.

Application

• β-Glucosidase Assays: As a chromogenic substrate for the specific detection and activity measurement of β-glucosidase enzymes.
• Diagnostic Reagents: A key component in clinical and industrial diagnostic kits for enzyme deficiency disorders or microbial identification.
• Microbiological Research: Used in microbial culture media for the differentiation of bacteria based on β-glucosidase production (e.g., in chromogenic agar).
• Enzyme Kinetics Studies: Employed in biochemical research to study enzyme mechanisms, inhibition, and kinetics.
• High-Throughput Screening (HTS): Utilized in pharmaceutical and agrochemical discovery for screening compound libraries against β-glucosidase targets.
• Plant Biochemistry: Applied in research to study glucosidase activity involved in plant defense mechanisms and secondary metabolism.
• Quality Control in Food & Beverage: Used in assays to monitor enzymatic activity relevant to processes in the food industry.

Basic Information

Product Name	3-Indoxyl-β-D-Glucopyranoside
CAS No.	487-60-5
Molecular Formula	C14H17NO6
Molecular Weight	295.29 g/mol
Synonyms	Indoxyl-β-D-glucoside; 3-Indolyl β-D-Glucopyranoside; Indoxyl Glucoside; 5-Indolyl β-D-Glucopyranoside; β-Glucosidase Substrate; X-Gluc (in plant histochemistry); 3-Indoxyl-β-D-glucoside; 1-(Indol-3-yl)-β-D-glucopyranose
EINECS	207-663-8

Quality Control

Our 3-Indoxyl-β-D-Glucopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, consider storage under desiccant or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.1%
Solubility	Clear, colorless solution in DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.