Description

H-Ala-Pro-Val-5-[(2-Aminoethyl)Amino]-Naphthalene-1-Sulfonic Acid is a specialized synthetic peptide derivative containing a naphthalene sulfonic acid fluorophore, designed for advanced biochemical research and diagnostic applications. This compound matters for its role as a highly sensitive and specific substrate or probe in enzymatic assays, particularly for proteases like dipeptidyl peptidase IV (DPP-IV). It is primarily needed by pharmaceutical R&D teams, diagnostic kit manufacturers, and academic research institutions focused on enzyme kinetics, inhibitor screening, and biomarker detection.

Application

• Protease Activity Assays: Acts as a fluorogenic substrate for the detection and quantification of dipeptidyl peptidase IV (DPP-IV/CD26) enzyme activity.
• Drug Discovery Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel DPP-IV inhibitors for diabetes and cancer research.
• Biochemical Research: Serves as a critical tool in studying enzyme kinetics, mechanism of action, and substrate specificity of proteases.
• Diagnostic Development: Employed in the development of clinical diagnostic assays and kits for measuring DPP-IV levels in biological samples.
• Cell Biology Studies: Utilized for in vitro and ex vivo studies to monitor DPP-IV activity in cell cultures and tissue samples.
• Quality Control in Biologics: Applied in the quality control testing of biopharmaceuticals where protease activity is a critical parameter.

Basic Information

Product Name	H-Ala-Pro-Val-5-[(2-Aminoethyl)Amino]-Naphthalene-1-Sulfonic Acid
CAS No.	396077-59-1
Molecular Formula	C₂₅H₃₃N₅O₆S
Molecular Weight	531.63 g/mol
Synonyms	Ala-Pro-Val-EDANS; APV-EDANS; H-Ala-Pro-Val-EDANS; EDANS-labeled Ala-Pro-Val tripeptide; 5-[(2-Aminoethyl)amino]naphthalene-1-sulfonyl-Ala-Pro-Val; Fluorogenic DPP-IV Substrate; DPP-IV Substrate, Fluorogenic; L-Alanyl-L-prolyl-L-valyl-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
EINECS	Contact for details

Quality Control

Our H-Ala-Pro-Val-5-[(2-Aminoethyl)Amino]-Naphthalene-1-Sulfonic Acid is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, MS for identity confirmation, and NMR for structural verification, to ensure it meets the high standards required for sensitive research applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, desiccate and keep frozen. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular mass
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.