Description

5-Bromo-4-Chloro-3-Indoxyl Phosphate, Dipotassium Salt is a high-purity chromogenic substrate widely used in enzymatic detection systems. This compound is critical for producing a vivid, insoluble blue precipitate upon enzymatic cleavage, making it an indispensable tool for sensitive and specific detection. It is primarily needed by manufacturers and researchers in the life sciences, diagnostic kit production, and molecular biology sectors for applications such as immunohistochemistry, Western blotting, and in situ hybridization.

Application

• Chromogenic Substrate for Alkaline Phosphatase (AP): A key component in AP-based detection systems for ELISA, Western blotting, and immunohistochemistry (IHC), producing a stable, intense blue color.
• Diagnostic Kit Manufacturing: Used in the formulation of clinical diagnostic kits for detecting specific antigens, antibodies, or nucleic acids.
• In Situ Hybridization (ISH): Employed as a substrate for labeling probes in ISH techniques to visualize gene expression in tissue sections.
• Histology and Pathology Staining: Provides high-contrast, permanent staining for cellular and tissue analysis in research and clinical pathology.
• Microbiology and Cell Biology: Used in assays to detect enzymatic activity of reporter genes or to study phosphatase activity in cells.
• Research & Development: Serves as a critical reagent in biochemical and pharmaceutical R&D for developing new assays and detection methodologies.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indoxyl Phosphate, Dipotassium Salt
CAS No.	390748-26-2
Molecular Formula	C8H5BrClNO4PK2
Molecular Weight	388.59 g/mol
Synonyms	BCIP, Dipotassium Salt; 5-Bromo-4-chloro-3-indolyl phosphate, dipotassium salt; 5-Bromo-4-chloro-1H-indol-3-yl dihydrogen phosphate, dipotassium salt; BCIP, K2 Salt; BCIP dipotassium; X-Phosphate, Dipotassium Salt; Alkaline Phosphatase Substrate, BCIP; Chromogenic AP Substrate
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indoxyl Phosphate, Dipotassium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to meet stringent specifications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. The product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO
Heavy Metals	≤ 20 ppm
Performance Test	Yields intense blue precipitate with AP enzyme

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.