Description

H-Lys-Thr-Glu-Glu-Ile-Ser-Glu-Val-Lys-Met-Pna is a synthetic peptide sequence featuring a para-nitroanilide (Pna) chromogenic group. This compound is a specialized substrate designed for the study and detection of specific protease enzyme activity, offering a reliable and quantifiable signal upon cleavage. It is primarily utilized by research institutions and pharmaceutical developers in the fields of biochemistry, enzymology, and drug discovery for kinetic assays and inhibitor screening.

Application

• Protease Enzyme Substrate: Used as a chromogenic substrate for the kinetic analysis and characterization of specific serine or cysteine proteases.
• Biochemical Research: Essential tool in academic and industrial research laboratories for studying enzyme mechanisms, specificity, and inhibition profiles.
• Drug Discovery Screening: Employed in high-throughput screening (HTS) assays to identify and evaluate potential protease inhibitors as therapeutic candidates.
• Diagnostic Development: Serves as a key component in the development of in vitro diagnostic tests for diseases where specific protease activity is a biomarker.
• Quality Control in Biologics: Can be used to monitor and ensure the absence or activity level of specific proteases in biopharmaceutical manufacturing processes.

Basic Information

Product Name	H-Lys-Thr-Glu-Glu-Ile-Ser-Glu-Val-Lys-Met-Pna
CAS No.	389867-93-0
Molecular Formula	C58H92N16O22S
Molecular Weight	1381.5 g/mol
Synonyms	Lys-Thr-Glu-Glu-Ile-Ser-Glu-Val-Lys-Met-pNA; KTEQISEVKM-pNA; H-Lys-Thr-Glu-Glu-Ile-Ser-Glu-Val-Lys-Met-4-nitroanilide; Peptide Substrate 389867-93-0; Chromogenic Peptide Substrate; Protease Substrate pNA; H-KTEQISEVKM-pNA; 4-Nitroanilide derivative of KTEQISEVKM
EINECS	Contact for details

Quality Control

Our peptide products are manufactured and analyzed under stringent quality systems to ensure high purity and batch-to-batch consistency. Each lot is subjected to comprehensive analytical testing including HPLC for purity, mass spectrometry (MS) for identity confirmation, and amino acid analysis (AAA). A comprehensive Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment to support your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 70%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.