Description

1,2-Dipalmitoyl-Sn-Glycero-3-Phosphoethanolamine-n-(7-Nitro-2-1,3-Benzoxadiazol-4-Yl) is a high-purity, fluorescently labeled phospholipid essential for advanced membrane biophysics and cellular research. Its core value lies in the NBD (7-Nitro-2-1,3-Benzoxadiazol-4-yl) fluorophore, which provides a sensitive, environmentally responsive probe for tracking lipid dynamics, membrane fusion, and intracellular trafficking. This compound is critically needed by researchers and formulators in pharmaceutical development, diagnostic assay design, and advanced lipid nanoparticle (LNP) characterization.

Application

• Membrane Biophysics Research: As a fluorescent tracer to study lipid bilayer properties, phase transitions, and lateral diffusion using techniques like FRAP (Fluorescence Recovery After Photobleaching) and FLIM (Fluorescence Lifetime Imaging Microscopy).
• Lipid Nanoparticle (LNP) & Liposome Development: To label and track the cellular uptake, biodistribution, and intracellular fate of drug delivery vehicles, particularly for mRNA and gene therapies.
• Cell Biology & Trafficking Studies: Visualization of endocytosis, exocytosis, and intracellular organelle dynamics by incorporating the probe into cellular membranes.
• Diagnostic Assay Development: Used in fluorescence-based assays to detect enzymatic activity (e.g., phospholipases) or to create sensitive biosensors.
• Model Membrane Systems: A key component in constructing artificial membranes (e.g., giant unilamellar vesicles or supported lipid bilayers) for controlled experimental systems.
• Protein-Lipid Interaction Analysis: Employed in fluorescence quenching or anisotropy studies to investigate how proteins bind to and modulate phospholipid membranes.

Basic Information

Product Name	1,2-Dipalmitoyl-Sn-Glycero-3-Phosphoethanolamine-n-(7-Nitro-2-1,3-Benzoxadiazol-4-Yl)
CAS No.	384823-46-5
Molecular Formula	C46H80N4O12P
Molecular Weight	912.11 g/mol
Synonyms	NBD-PE; 1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl); DPPE-NBD; NBD-DPPE; N-(7-Nitro-2,1,3-benzoxadiazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine; Fluorescent PE; NBD Phosphatidylethanolamine; 16:0 NBD PE
EINECS	Contact for details

Quality Control

Our 1,2-Dipalmitoyl-Sn-Glycero-3-Phosphoethanolamine-n-(7-Nitro-2-1,3-Benzoxadiazol-4-Yl) is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency critical for sensitive research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity, mass spectrometry for identity confirmation, and fluorescence spectroscopy to verify performance. Certificates of Analysis (COA) with detailed chromatograms and spectral data are provided to support your research and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store sealed vials under an inert atmosphere (argon or nitrogen) in a freezer. Allow the sealed container to reach room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC-MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Fluorescence Properties	Excitation ~460 nm, Emission ~534 nm (in methanol)
Solubility	Soluble in chloroform, methanol, DMSO; forms vesicles in aqueous buffers
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.