Description

(1-Oxyl-2,2,5,5-Tetramethyl-3-Pyrroline-3-Methyl) Methanethiosulfonate-15N-D15 is a highly specialized, isotopically labeled spin-labeling reagent used for advanced site-directed spin labeling (SDSL) in structural biology. Its core value lies in providing exceptional sensitivity and resolution for probing protein dynamics, conformational changes, and molecular interactions through Electron Paramagnetic Resonance (EPR) spectroscopy. This compound is essential for researchers and developers in biopharmaceutical R&D, structural biology, and advanced materials science who require precise, non-perturbing probes for studying complex biomolecular systems.

Application

• Site-Directed Spin Labeling (SDSL) for EPR Spectroscopy: Primary application for covalent attachment of a stable nitroxide radical to specific cysteine residues in proteins and peptides.
• Protein Structure & Dynamics Analysis: Mapping secondary structure, measuring distances, and monitoring conformational changes in soluble and membrane proteins.
• Membrane Protein Studies: Investigating the topology, folding, and lipid interactions of integral membrane proteins.
• Nucleic Acid Research: Probing the structure and dynamics of DNA and RNA by labeling modified nucleotides.
• Drug Discovery & Biophysics: Characterizing protein-ligand and protein-protein interactions for target validation and mechanism-of-action studies.
• DEER/PELDOR Distance Measurements: Enabling precise nanometer-scale distance measurements in biomacromolecules using pulsed EPR techniques.
• Advanced Materials Characterization: Serving as a spin probe for studying polymer dynamics, surface modifications, and self-assembly processes.

Basic Information

Item	Detail
Product Name	(1-Oxyl-2,2,5,5-Tetramethyl-3-Pyrroline-3-Methyl) Methanethiosulfonate-15N-D15
CAS No.	384342-57-8
Molecular Formula	C11H8D1515N1O3S2
Molecular Weight	Contact for details
Synonyms	MTSSL-15N-D15; MTSL-15N-D15; (1-Oxyl-2,2,5,5-tetramethyl-δ3-pyrroline-3-methyl) Methanethiosulfonate-15N-D15; S-(1-Oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl methanesulfonothioate-15N-D15; Isotopically Labeled MTS Spin Label; 15N, D15-MTSSL; 15N, D15-MTSL; Nitroxide Spin Label-15N-D15
EINECS	Contact for details

Quality Control

Our isotopically labeled spin-labeling reagents are produced and tested under stringent conditions to ensure the highest levels of isotopic enrichment, chemical purity, and radical stability. Each batch is characterized by advanced analytical techniques including HPLC, NMR (1H, 13C), and mass spectrometry to confirm identity, isotopic purity (>98 atom% 15N, >98 atom% D), and chemical purity. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your research integrity and documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) to minimize oxidation and ensure long-term stability of the nitroxide radical. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation. For prolonged storage, keep desiccated.

Specification

Item	Specification
Appearance	Orange to red solid or oil
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Purity (15N)	≥ 98 atom%
Isotopic Purity (D)	≥ 98 atom% D
Solubility	Soluble in common organic solvents (DMSO, ethanol, acetonitrile)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.