Description

4-Nitrophenyl 2-o-(A-L-Fucopyranosyl)-A-D-Galactopyranoside is a specialized synthetic glycoside derivative, serving as a critical chromogenic substrate for enzymatic assays. Its core value lies in enabling precise, colorimetric detection and quantification of specific glycosidase enzyme activity, which is fundamental to biochemical research and diagnostic development. This high-purity compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic reagent industries who require reliable tools for enzyme kinetics, inhibitor screening, and quality control testing.

Application

• Glycosidase Enzyme Assays: Primary chromogenic substrate for the detection and kinetic analysis of specific α-galactosidases and related enzymes.
• Biochemical Research: Used in fundamental studies of carbohydrate metabolism, enzyme mechanisms, and protein-glycan interactions.
• Diagnostic Reagent Manufacturing: A key component in the formulation of kits for the diagnosis of genetic disorders like Fabry disease, which involves α-galactosidase A deficiency.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize potential glycosidase inhibitors for drug discovery.
• Quality Control (QC) Testing: Used by biopharmaceutical companies to monitor the activity and consistency of enzyme-based therapeutics or processes.
• Microbiology Studies: Applied in research to study bacterial glycosidases and their role in pathogenesis or carbohydrate utilization.

Basic Information

Product Name	4-Nitrophenyl 2-o-(A-L-Fucopyranosyl)-A-D-Galactopyranoside
CAS No.	383417-46-7
Molecular Formula	C18H25NO12
Molecular Weight	447.39 g/mol
Synonyms	4-Nitrophenyl 2-O-α-L-Fucopyranosyl-α-D-galactopyranoside; p-Nitrophenyl 2-O-α-L-fucopyranosyl-α-D-galactopyranoside; 2'-Fucosyllactose 4-nitrophenyl glycoside analog; α-Galactosidase substrate (specific); PNPGal-α-Fuc; 4-NP-α-D-Galp-(1→2)-α-L-Fucp; Fabry disease diagnostic substrate
EINECS	Contact for details

Quality Control

Every batch of 4-Nitrophenyl 2-o-(A-L-Fucopyranosyl)-A-D-Galactopyranoside is manufactured under a strict quality management system and undergoes comprehensive analytical testing to ensure identity, purity, and performance suitability. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes data from HPLC, NMR, and mass spectrometry analysis. Our quality commitment aligns with the standards required for research and in-vitro diagnostic (IVD) use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccation. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO, DMF
Enzymatic Activity	Suitable for α-galactosidase assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.