Description

4-Methylumbelliferylb-D-Gentiotrioside is a specialized fluorogenic glycosidase substrate used in enzymatic assays and biochemical research. This compound is valued for its high sensitivity and specificity in detecting and quantifying enzyme activity, particularly for β-glucosidase and related enzymes. It is an essential reagent for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries seeking precise analytical tools.

Application

• Enzymatic Assay Substrate: Primary use as a fluorogenic substrate for the detection and kinetic study of β-glucosidase and cellulase enzymes.
• Biochemical Research: Tool for investigating glycosidase mechanisms, inhibitor screening, and enzyme characterization in academic and industrial R&D.
• Diagnostic Reagent Manufacturing: Key component in the formulation of diagnostic kits for genetic disorders like Gaucher's disease, which involve glucocerebrosidase deficiency.
• Pharmaceutical Quality Control: Used in potency assays for enzyme replacement therapies and in-process testing during biomanufacturing.
• Microbiology & Plant Biology: Applied in studies of microbial cellulolytic systems and plant cell wall degradation processes.
• High-Throughput Screening (HTS): Suitable for fluorometric HTS platforms in drug discovery to identify novel glycosidase modulators.

Basic Information

Product Name	4-Methylumbelliferylb-D-Gentiotrioside
CAS No.	383160-16-5
Molecular Formula	C34H46O21
Molecular Weight	790.72 g/mol
Synonyms	4-Methylumbelliferyl-β-D-gentiotrioside; 4-MU-β-gentiotrioside; 4-Methylumbelliferyl β-gentiotrioside; 4-MUGT; 4-Methylumbelliferyl β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside; Fluorogenic β-glucosidase substrate; 4-MU-β-Glc-(1,6)-β-Glc-(1,6)-β-Glc
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferylb-D-Gentiotrioside is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for critical research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific activity validation in enzymatic assays. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all specifications and test results.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or light may affect performance. For long-term stability, consider storage under inert atmosphere or with desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Optical Rotation	Contact for details
Fluorogenic Activity	Validated with reference enzyme
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.