Description

Bodipy 630/650-X NHS Ester (Succinimidyl Ester) is a high-performance, reactive fluorescent dye derivative designed for labeling biomolecules. This compound is essential for creating stable, bright conjugates for advanced detection and imaging applications. It is primarily needed by researchers and manufacturers in the fields of bioconjugation, flow cytometry, fluorescence microscopy, and in vitro diagnostics.

Application

• Bioconjugation and Probe Development: Covalent labeling of proteins, antibodies, peptides, and oligonucleotides via primary amine groups.
• Flow Cytometry: Creation of bright antibody-dye conjugates for multi-color cell analysis and immunophenotyping.
• Fluorescence Microscopy & Imaging: Preparation of fluorescent probes for cellular and tissue imaging with excellent photostability.
• Diagnostic Assays: Used in the development of fluorescent immunoassays (FLISA) and other clinical diagnostic reagents.
• Biomolecular Tracking: Studying protein-protein interactions, localization, and dynamics in live or fixed cells.
• Materials Science: Functionalization of nanoparticles and polymers for sensing and bioimaging applications.

Basic Information

Product Name	Bodipy 630/650-X NHS Ester (Succinimidyl Ester)
CAS No.	380367-48-6
Molecular Formula	C24H28BF2N3O5
Molecular Weight	503.31 g/mol
Synonyms	BODIPY® 630/650-X, SE; BDP 630/650-X NHS ester; 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Propionic Acid, Succinimidyl Ester; BODIPY® FL NHS Ester (630/650-X); Boron Dipyrromethene Dye NHS Ester; BODIPY® 650/665-X, SE; 3-(2,6-Dimethyl-4-bora-3a,4a-diaza-s-indacen-8-yl)propionic Acid N-Hydroxysuccinimide Ester
EINECS	Contact for details

Quality Control

Our Bodipy 630/650-X NHS Ester is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and reactivity. Certificates of Analysis (COA) with detailed spectral data are provided to support your research and development.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Red to dark red solid or film
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Extinction Coefficient (ε)	~90,000 cm-1M-1 at ~646 nm
Excitation/Emission Maxima	~630 nm / ~650 nm
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	< 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.