Description

2-Phenyl-3-(4-Nitrophenyl)-5-Methyltetrazolium Chloride is a specialized tetrazolium salt derivative, primarily recognized for its role as a vital biochemical reagent. Its core value lies in its ability to serve as a sensitive redox indicator in enzymatic assays, particularly for detecting cellular metabolic activity. This compound is essential for researchers and quality control professionals in the pharmaceutical, biotechnology, and life sciences industries, where precise measurement of dehydrogenase activity and cell viability is critical.

Application

• Cell Viability and Cytotoxicity Assays: Used as a key substrate in MTT and related assays to measure mitochondrial activity in living cells.
• Enzymatic Activity Detection: Serves as an electron acceptor in dehydrogenase enzyme assays, forming a colored formazan product for spectrophotometric quantification.
• Microbiological Testing: Applied in microbial identification and metabolic studies to assess bacterial and fungal growth and respiration.
• Pharmaceutical Research & Development: Utilized in high-throughput screening (HTS) for drug discovery to evaluate the effects of novel compounds on cell metabolism.
• Biochemical Research: Acts as a diagnostic tool in biochemistry and molecular biology laboratories for studying oxidative stress and metabolic pathways.
• Quality Control in Cell Culture: Employed to monitor the health and proliferation rates of cell lines in biomanufacturing and academic research settings.

Basic Information

Product Name	2-Phenyl-3-(4-Nitrophenyl)-5-Methyltetrazolium Chloride
CAS No.	376353-99-0
Molecular Formula	C20H16N6O2•Cl
Molecular Weight	407.84 g/mol
Synonyms	4-Nitrophenyl Tetrazolium Chloride Derivative; 5-Methyl-2-phenyl-3-(4-nitrophenyl)tetrazolium Chloride; MTT Tetrazolium Analog; Tetrazolium Red Salt; Nitrophenyl Tetrazolium Salt; Cell Viability Assay Reagent; Dehydrogenase Assay Substrate
EINECS	Contact for details

Quality Control

Our 2-Phenyl-3-(4-Nitrophenyl)-5-Methyltetrazolium Chloride is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive biochemical applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed in a desiccated environment away from direct light exposure to maintain stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, Methanol; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.