Description

5-Bromo-4-Chloro-3-Indolyl β-D-Glucuronide Sodium Salt is a chromogenic substrate widely used in enzymatic assays for the detection of β-glucuronidase activity. This compound is critical for research and diagnostic applications where precise and sensitive enzyme detection is required. It is primarily utilized by professionals in molecular biology, microbiology, and diagnostic kit manufacturing for applications such as gene reporter assays and bacterial identification.

Application

• Gene Reporter Assays: A key substrate for detecting β-glucuronidase (GUS) reporter gene expression in plant and microbial genetic engineering.
• Microbiological Testing: Used for the specific identification of E. coli and other β-glucuronidase-producing bacteria in food, water, and clinical samples.
• Diagnostic Kits: An essential component in commercial diagnostic kits and reagents for enzymatic activity measurement.
• Histochemistry & Cell Biology: Employed in histological staining to localize β-glucuronidase activity in tissue sections and cell cultures.
• Enzyme Inhibition Studies: Serves as a substrate for screening and characterizing potential β-glucuronidase inhibitors in drug discovery.
• Quality Control in Biotechnology: Used in QC testing of recombinant proteins and fermentation processes involving GUS-tagged products.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl β-D-Glucuronide Sodium Salt
CAS No.	370100-64-4
Molecular Formula	C14H13BrClNNaO7
Molecular Weight	454.60 g/mol
Synonyms	X-Glucuronide Sodium Salt; 5-Bromo-4-chloro-3-indolyl-β-D-glucopyranosiduronic acid sodium salt; BCIG; X-GlcA; X-β-D-Glucuronide; 5-Bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid sodium salt; Sodium 5-Bromo-4-chloro-3-indolyl-β-D-glucuronide
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl β-D-Glucuronide Sodium Salt is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). For long-term stability, store desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water
Heavy Metals	< 10 ppm
Biological Activity	Suitable for enzyme substrate use

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.