Description

(+)-Biotin-Peg12-Ch2Ch2Coonhs Ester is a high-purity, heterobifunctional PEG linker designed for advanced bioconjugation and chemical biology applications. This compound is critical for creating stable, site-specific conjugates between biotinylated molecules and amine-containing targets, enabling precise labeling and immobilization strategies. It is primarily needed by researchers and manufacturers in the pharmaceutical, diagnostic, and life science sectors for developing targeted therapeutics, diagnostic assays, and affinity purification systems.

Application

• Bioconjugation Reagent: For the site-specific coupling of biotinylated probes, peptides, or antibodies to amine-functionalized surfaces, nanoparticles, or other biomolecules.
• Affinity Chromatography Ligand Synthesis: Used to functionalize resins or beads with biotin for streptavidin/avidin-based purification systems.
• Diagnostic Assay Development: Enables the creation of sensitive ELISA, lateral flow, or other immunoassay formats by linking detection antibodies or antigens to biotin.
• Drug Delivery & ADC (Antibody-Drug Conjugate) Research: Serves as a key building block in the synthesis of targeted drug delivery systems and ADCs, utilizing the long, flexible PEG12 spacer to improve solubility and reduce aggregation.
• Surface Plasmon Resonance (SPR) & Biosensor Fabrication: For immobilizing biotinylated ligands onto sensor chips to study biomolecular interactions.
• Cell Labeling & Imaging: Facilitates the labeling of cells or proteins with biotin for subsequent detection with fluorescent streptavidin conjugates in microscopy and flow cytometry.
• Protein & Nucleic Acid Modification: Used to introduce biotin tags onto proteins or oligonucleotides for pull-down assays, sequencing, or microarray applications.

Basic Information

Product Name	(+)-Biotin-Peg12-Ch2Ch2Coonhs Ester
CAS No.	365441-71-0
Molecular Formula	C58H108N4O27S
Molecular Weight	~1349.6 g/mol
Synonyms	Biotin-PEG12-NHS Ester; Biotin-PEG12-NHS; Biotin-PEG12-Succinimidyl Ester; Biotinamidohexanoyl-PEG12-NHS Ester; Biotin-PEG12-NHS Ester, (+)-; NHS-PEG12-Biotin; N-Hydroxysuccinimide ester of Biotin-PEG12-acid; Biotinylated PEG NHS Ester (PEG12); EZ-Link™ Biotin-PEG12-NHS Ester (analog); 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoylamino)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanoate
EINECS	Contact for details

Quality Control

Our (+)-Biotin-Peg12-Ch2Ch2Coonhs Ester is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS). We provide a detailed Certificate of Analysis (COA) with each shipment, documenting critical parameters such as assay purity, functional group activity, and absence of critical impurities. Our quality commitment supports research and GMP-grade development workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and the NHS ester group is susceptible to hydrolysis. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (Functional NHS Ester)	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.