Description

n-Acetyl-5-[1,2-Bis(Acetyloxy)Ethyl]-5'-o-Dmt-2'-Deoxycytidine 3'-Ce Phosphoramidite is a high-purity, protected phosphoramidite monomer specifically engineered for oligonucleotide synthesis. This compound is critical for the solid-phase synthesis of modified DNA and RNA strands, enabling the precise incorporation of functionalized nucleosides. It is an essential raw material for research and development in therapeutic oligonucleotides, antisense technology, and diagnostic probe development.

Application

• Solid-Phase Oligonucleotide Synthesis: Key building block for automated DNA/RNA synthesizers to produce modified oligonucleotides.
• Therapeutic Oligonucleotide Manufacturing: Synthesis of antisense oligonucleotides (ASOs), siRNA, and aptamers for pharmaceutical applications.
• Diagnostic Probe Development: Creation of labeled probes for genetic testing, fluorescence in situ hybridization (FISH), and microarray technologies.
• Basic Research: Tool for studying nucleic acid structure, function, and protein-nucleic acid interactions with site-specific modifications.
• Synthesis of Primers and Linkers: Production of specialized primers for PCR and next-generation sequencing (NGS) platforms.
• Bioconjugation: Intermediate for attaching oligonucleotides to surfaces, nanoparticles, or other biomolecules.

Basic Information

Product Name	n-Acetyl-5-[1,2-Bis(Acetyloxy)Ethyl]-5'-o-Dmt-2'-Deoxycytidine 3'-Ce Phosphoramidite
CAS No.	364613-34-3
Molecular Formula	C46H55N4O12P
Molecular Weight	887.93 g/mol
Synonyms	5-[1,2-Bis(acetyloxy)ethyl]-N-acetyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); 5-(1,2-Diacetoxyethyl)-N-acetyl-5'-O-DMT-2'-deoxycytidine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); CE Phosphoramidite of 5-(1,2-Diacetoxyethyl)-N-acetyl-5'-O-DMT-2'-deoxycytidine; Modified dC Phosphoramidite; 5-(1,2-Diacetoxyethyl)dC-CE Phosphoramidite; Acetylated and DMT-Protected Cytidine Phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of our phosphoramidite monomers is produced under strict quality management systems. We employ advanced analytical techniques including HPLC, 31P NMR, and 1H NMR to ensure chemical identity, purity, and stability. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and performance in coupling efficiency tests is provided with each shipment to guarantee consistency for your critical synthesis workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and strictly light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Moisture (Karl Fischer)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.