Description

Z-Leu-Arg-4Mβna is a specialized fluorogenic peptide substrate designed for the sensitive detection and quantification of protease activity. This compound is critical for biochemical research and pharmaceutical development, enabling precise enzymatic assays. It is primarily utilized by researchers and quality control laboratories in the life sciences, biotechnology, and pharmaceutical industries for studying enzyme kinetics and screening potential therapeutic inhibitors.

Application

• Protease Activity Assays: As a selective fluorogenic substrate for trypsin-like serine proteases, including thrombin, plasmin, and urokinase-type plasminogen activator (uPA).
• Drug Discovery & Screening: High-throughput screening (HTS) of compound libraries to identify novel protease inhibitors for therapeutic development.
• Biochemical Research: Kinetic analysis of enzyme mechanisms, determination of Michaelis-Menten constants (Km), and investigation of catalytic efficiency.
• Diagnostic Development: A key reagent in the development of in vitro diagnostic (IVD) kits for detecting and monitoring protease-related pathologies.
• Quality Control in Biologics: Monitoring the activity and stability of protease enzymes used in biomanufacturing processes.

Basic Information

Product Name	Z-Leu-Arg-4Mβna
CAS No.	361544-52-7
Molecular Formula	C27H41N7O7
Molecular Weight	575.66 g/mol
Synonyms	Benzyloxycarbonyl-Leucyl-Arginine 4-Methoxy-β-naphthylamide; Z-Leu-Arg-MNA; Z-Leu-Arg-4-Methoxy-2-naphthylamide; Carbobenzoxy-Leu-Arg-4-Methoxy-β-naphthylamide; Nα-CBZ-Leucyl-arginine 4-methoxy-β-naphthylamide; Fluorogenic Substrate for Trypsin; Thrombin Substrate; uPA Substrate
EINECS	Contact for details

Quality Control

Our Z-Leu-Arg-4Mβna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by mass spectrometry. Certificates of Analysis (COA) detailing batch-specific results are provided to ensure traceability and compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.