Description

E-Maleimidopropionic Acid Hydrazide is a heterobifunctional crosslinking reagent featuring a maleimide group and a hydrazide group. This compound is essential for creating stable, site-specific conjugations between biomolecules, such as antibodies and enzymes, and various probes or solid supports. It is a critical reagent for researchers and manufacturers in the fields of bioconjugation, diagnostic assay development, and targeted drug delivery systems.

Application

• Bioconjugation Reagent: For the covalent coupling of peptides, proteins, and antibodies to other molecules via the maleimide-thiol and hydrazide-aldehyde chemistry.
• Diagnostic Assay Development: Used in the preparation of enzyme-labeled antibodies and antigens for ELISA, lateral flow assays, and other immunoassays.
• ADC (Antibody-Drug Conjugate) Linker Synthesis: Serves as a key intermediate in the design and synthesis of linkers for targeted cancer therapeutics.
• Surface Functionalization: Immobilization of biomolecules onto activated surfaces, beads, or nanoparticles for biosensors and affinity purification.
• Fluorescent Probe Labeling: Conjugation of fluorescent dyes to targeting molecules for cellular imaging and flow cytometry.
• Polymer Modification: Introduction of reactive handles into polymers for the creation of functional biomaterials.

Basic Information

Product Name	E-Maleimidopropionic Acid Hydrazide
CAS No.	359436-60-5
Molecular Formula	C7H9N3O3
Molecular Weight	183.16 g/mol
Synonyms	3-Maleimidopropionic Acid Hydrazide; Maleimidopropanoic Acid Hydrazide; 3-(2,5-Dihydro-2,5-dioxo-1H-pyrrol-1-yl)propanehydrazide; 1-(3-Hydrazinyl-3-oxopropyl)-1H-pyrrole-2,5-dione; MPBH; EMCS Hydrazide; Maleimide-PEG3-Hydrazide (core structure)
EINECS	Contact for details

Quality Control

Our E-Maleimidopropionic Acid Hydrazide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by IR and NMR spectroscopy, to ensure it meets the high standards required for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.