Description

Ac-Ala-Amc is a specialized fluorogenic peptide substrate used extensively in biochemical and pharmaceutical research. This compound is critical for the sensitive detection and quantification of protease enzyme activity, particularly for enzymes like calpain. It is an essential tool for researchers and developers in the fields of drug discovery, diagnostic assay development, and proteomics, where precise enzymatic measurement is required.

Application

• Protease Activity Assays: Primary substrate for fluorometric assays measuring calpain and related cysteine protease activity.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and evaluate potential protease inhibitors as therapeutic candidates.
• Biochemical Research: A key reagent in studying protease kinetics, enzyme mechanisms, and signal transduction pathways involving proteolytic cleavage.
• Diagnostic Development: Employed in the development of sensitive diagnostic kits for diseases where protease levels are a biomarker.
• Cell Biology Studies: Used to monitor intracellular protease activity in live-cell imaging and apoptosis research.
• Quality Control in Biologics: Applied in the characterization and potency testing of biopharmaceuticals that involve protease components.

Basic Information

Product Name	Ac-Ala-Amc
CAS No.	355137-87-0
Molecular Formula	C₁₅H₁₇N₃O₄
Molecular Weight	303.31 g/mol
Synonyms	N-Acetyl-Ala-AMC; Acetyl-Alanine-7-Amido-4-Methylcoumarin; Ac-Ala-AMC; N-Acetyl-L-alanine 7-amido-4-methylcoumarin; Calpain Substrate I; Suc-LLVY-AMC (related class); Fluorogenic Calpain Substrate; Ac-Ala-NH-Mec
EINECS	Contact for details

Quality Control

Every batch of Ac-Ala-Amc is manufactured and tested under strict quality management systems. We guarantee high-purity material suitable for sensitive research applications, with comprehensive testing including HPLC purity analysis, identity confirmation (IR/MS), and functional activity validation. Certificates of Analysis (COA) detailing all specifications and test results are provided with each shipment to ensure traceability and compliance with your research protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Functional Activity	≥ 95% of reference standard
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.