Description

Ac-Leu-Val-Cys-Asp-Amc CAS NO 354151-86-3 is a synthetic, fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound matters for researchers and quality control labs requiring precise measurement of specific protease activity, such as caspase-3 and related enzymes. It is primarily needed by the pharmaceutical, biotechnology, and academic research sectors for drug discovery, apoptosis studies, and biochemical pathway analysis.

Application

• Biochemical Research: As a fluorogenic substrate for studying caspase-3, caspase-7, and other cysteine proteases involved in apoptosis and inflammation.
• Drug Discovery & Screening: High-throughput screening (HTS) of potential therapeutic compounds that modulate protease activity in disease models.
• Diagnostic Assay Development: A key component in the development of sensitive in vitro diagnostic (IVD) kits for detecting specific enzymatic biomarkers.
• Cell Biology Studies: Monitoring programmed cell death (apoptosis) in real-time within live cells or cell lysates.
• Enzyme Kinetics & Characterization: Determining kinetic parameters (Km, Vmax) and inhibition constants (Ki) for target proteases.
• Quality Control in Biologics: Testing for the presence and activity of specific proteases during the manufacturing of biopharmaceuticals.

Basic Information

Item	Details
Product Name	Ac-Leu-Val-Cys-Asp-Amc
CAS No.	354151-86-3
Molecular Formula	C30H41N7O9S
Molecular Weight	675.75 g/mol
Synonyms	Ac-Leu-Val-Cys-Asp-7-Amino-4-methylcoumarin; Ac-LVCD-Amc; Caspase-3 Substrate I (Ac-DEVD-Amc analog); N-Acetyl-Leu-Val-Cys-Asp-AMC; Ac-Leu-Val-Cys-Asp-AMC; Fluorogenic Caspase Substrate; Ac-L-V-C-D-AMC; N-Acetyl-L-leucyl-L-valyl-L-cysteinyl-L-aspartyl-7-amido-4-methylcoumarin
EINECS	Contact for details

Quality Control

Our Ac-Leu-Val-Cys-Asp-Amc is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.