Description

n-Biotinylcaproylaminoethyl Methanethiosulfonate is a specialized bioconjugation reagent designed for the site-specific labeling of proteins and other biomolecules. This compound matters for researchers and developers requiring high-precision, covalent attachment of biotin tags via disulfide bond formation, enabling downstream detection, purification, or immobilization. It is primarily needed by biotechnology, pharmaceutical, and diagnostic industries for applications in proteomics, assay development, and targeted drug delivery systems.

Application

• Site-Specific Protein Biotinylation: Covalent attachment of biotin to cysteine residues for streptavidin-based assays and pull-down experiments.
• Proteomics Research: Labeling of peptides and proteins for mass spectrometry analysis and protein interaction studies.
• Diagnostic Assay Development: Conjugation of detection enzymes or antibodies in ELISA and lateral flow immunoassays.
• Affinity Chromatography Ligand Immobilization: Functionalization of solid supports for the purification of target biomolecules.
• Drug Conjugate Synthesis: As a linker-payload component in the development of antibody-drug conjugates (ADCs) and other targeted therapeutics.
• Cell Surface Labeling: Tagging of membrane proteins for fluorescence-activated cell sorting (FACS) or microscopy.
• Nanoparticle Functionalization: Biotinylation of nanoparticles for targeted delivery and imaging applications.

Basic Information

Product Name	n-Biotinylcaproylaminoethyl Methanethiosulfonate
CAS No.	353754-95-7
Molecular Formula	C19H33N3O5S3
Molecular Weight	495.67 g/mol
Synonyms	Biotin-HPDP; Biotinylated HPDP; N-(6-(Biotinamido)hexyl)-3'-(2'-pyridyldithio)propionamide; Biotin-HPDP Crosslinker; 3-(2-Pyridyldithio)propionyl biocytin; Biotin-LC-HPDP; N-[6-(Biotinamido)hexyl]-3-(2-pyridyldithio)propionamide; EZ-Link Biotin-HPDP
EINECS	Contact for details

Quality Control

Our n-Biotinylcaproylaminoethyl Methanethiosulfonate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance. We support compliance with relevant research and development standards, and specifications can be tailored to meet specific project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in ethanol
Functional Activity	Conforms (Biotin binding assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.