Description

Biocytinamidoethyl Methanethiosulfonate, Trifluoroacetic Acid Salt is a high-purity, chemically modified biotin derivative designed for advanced bioconjugation and labeling applications. This compound is engineered to provide a reactive methanethiosulfonate (MTS) group, enabling highly specific and stable covalent attachment to cysteine thiol groups in proteins and peptides under mild conditions. It is an essential research-grade reagent for scientists in pharmaceutical R&D, proteomics, and structural biology who require precise and irreversible site-directed labeling.

Application

• Site-Directed Protein Labeling: Covalent attachment of biotin tags to specific cysteine residues for purification, detection, or immobilization.
• Proteomics Research: Biotinylation of proteins for pull-down assays, affinity chromatography, and mass spectrometry analysis.
• Structural Biology: Introduction of biotin probes for electron microscopy (EM) and X-ray crystallography studies.
• Diagnostic Assay Development: Conjugation of detection molecules (e.g., enzymes, fluorophores) to antibodies or other proteins in immunoassay platforms.
• Drug Discovery: Creation of biotinylated drug candidates or targets for high-throughput screening (HTS) and binding studies.
• Bioconjugate Synthesis: A key intermediate for synthesizing custom biotinylated probes, linkers, and molecular tools.

Basic Information

Item	Detail
Product Name	Biocytinamidoethyl Methanethiosulfonate, Trifluoroacetic Acid Salt
CAS No.	353754-92-4
Molecular Formula	C18H30F3N5O7S2
Molecular Weight	549.59 g/mol
Synonyms	Biotinamidoethyl Methanethiosulfonate TFA Salt; Biotin-MTS; Biotin-PEG2-MTS TFA; N-Biotinyl-2-aminoethyl methanethiosulfonate; 2-((Biotinamido)ethyl) methanethiosulfonate trifluoroacetate; Biocytin-MTS; Biotinamidoethyl MTS; Biotinylated Methanethiosulfonate Reagent
EINECS	Contact for details

Quality Control

Every batch of Biocytinamidoethyl Methanethiosulfonate, Trifluoroacetic Acid Salt is manufactured under strict quality protocols to ensure the high purity and consistency required for sensitive research applications. Our quality assurance includes comprehensive analytical testing by HPLC, NMR, and mass spectrometry to verify identity, purity, and absence of critical impurities. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming compliance with our stringent in-house specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive). For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.