Description

Glutaryl-Phe-Ala-Ala-Phe-Amc CAS NO 351858-67-8 is a synthetic, fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of protease activity, particularly elastase and related enzymes. It is an essential tool for pharmaceutical R&D, biochemical research, and diagnostic kit development where accurate kinetic analysis is paramount.

Application

• Protease Activity Assays: Primary substrate for the fluorometric determination of human leukocyte elastase (HLE), pancreatic elastase, and other serine proteases.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) to identify and characterize novel elastase inhibitors for therapeutic applications in conditions like chronic obstructive pulmonary disease (COPD) and cystic fibrosis.
• Biochemical Research: Enables kinetic studies of enzyme mechanisms, specificity profiling, and the investigation of protease function in biological pathways.
• Diagnostic Development: A key component in the formulation of diagnostic kits for measuring elastase levels in clinical samples, aiding in disease monitoring and research.
• Quality Control in Biomanufacturing: Employed to monitor and validate the activity of enzyme-based biopharmaceuticals during production and purification processes.

Basic Information

Product Name	Glutaryl-Phe-Ala-Ala-Phe-Amc
CAS No.	351858-67-8
Molecular Formula	C41H48N6O9
Molecular Weight	768.86 g/mol
Synonyms	Glutaryl-Phenylalanyl-Alanyl-Alanyl-Phenylalanyl-7-Amino-4-Methylcoumarin; Glutaryl-F-A-A-F-AMC; MeOSuc-AAPV-AMC (common related analog); Suc-AAPF-AMC; Elastase Substrate IV; Fluorogenic Elastase Substrate; HLE Substrate; 7-Amino-4-methylcoumarin conjugate of Glutaryl-Phe-Ala-Ala-Phe.
EINECS	Contact for details

Quality Control

Our Glutaryl-Phe-Ala-Ala-Phe-Amc is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substances is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.