Description

Z-Leu-Leu-Glu-Amc CAS NO 348086-66-8 is a fluorogenic peptide substrate specifically designed for the sensitive detection and quantification of proteolytic enzyme activity. This compound is a critical tool for biochemical research, enabling the study of protease kinetics, inhibitor screening, and cellular signaling pathways. It is primarily utilized by researchers and scientists in the fields of pharmaceutical development, biochemistry, and molecular biology for high-throughput assays and mechanistic studies.

Application

• Protease Activity Assays: As a fluorogenic substrate for various proteases, including calpains and other cysteine proteases.
• Drug Discovery Screening: High-throughput screening (HTS) of potential protease inhibitors for therapeutic development.
• Biochemical Research: Kinetic analysis and characterization of enzyme mechanisms and specificity.
• Cell Biology Studies: Monitoring protease activity in cell lysates and live-cell imaging applications.
• Diagnostic Development: A key component in developing in vitro diagnostic tests for diseases involving aberrant protease activity.
• Quality Control in Biologics: Testing for residual protease activity in biopharmaceutical manufacturing processes.

Basic Information

Product Name	Z-Leu-Leu-Glu-Amc
CAS No.	348086-66-8
Molecular Formula	C30H44N4O9
Molecular Weight	604.70 g/mol
Synonyms	Z-LLE-AMC; N-CBZ-Leu-Leu-Glu-7-amido-4-methylcoumarin; CBZ-Leu-Leu-Glutamic Acid AMC; Calpain Substrate I; Suc-LLVY-AMC (structurally related analog); Fluorogenic Calpain Substrate; 7-Amido-4-methylcoumarin conjugate of Z-Leu-Leu-Glu; N-α-Carbobenzoxy-L-leucyl-L-leucyl-L-glutamic acid 7-amido-4-methylcoumarin
EINECS	Contact for details

Quality Control

Our Z-Leu-Leu-Glu-Amc is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and spectral analysis (NMR, MS) for structural confirmation, ensuring it meets the high standards required for sensitive research applications. Certificates of Analysis (COA) with detailed chromatograms and spectral data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.