Description

5-Bromo-4-Chloro-3-Indolyl-α-D-Maltotriose is a specialized chromogenic substrate used in enzymatic assays and diagnostic applications. Its core value lies in its ability to produce a distinct, insoluble blue-colored precipitate upon cleavage by specific glycosidase enzymes, enabling precise detection and quantification. This compound is essential for researchers and manufacturers in the life sciences, particularly for developing diagnostic kits, studying carbohydrate metabolism, and conducting microbiological identification.

Application

• Diagnostic Reagent Manufacturing: A key component in chromogenic media and test kits for the detection of α-glucosidase and related enzyme activities in clinical microbiology.
• Enzymology Research: Used as a specific substrate to study the kinetics, inhibition, and specificity of α-glucosidase and other carbohydrate-processing enzymes.
• Microbiological Identification: Incorporated into selective agar plates for the differentiation and identification of bacterial species (e.g., certain *Enterobacteriaceae*) based on their enzymatic profiles.
• Biochemical Assay Development: Serves as a critical reagent in high-throughput screening (HTS) assays for drug discovery targeting glycosidase enzymes.
• Food & Beverage Quality Control: Applied in enzymatic methods to analyze carbohydrate content or detect microbial contamination in samples.
• Plant & Agricultural Research: Utilized to investigate glycosidase activity in plant physiology and pathology studies.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl-α-D-Maltotriose
CAS No.	341972-94-9
Molecular Formula	C30H41BrClNO21
Molecular Weight	851.00 g/mol
Synonyms	X-α-D-Maltotriose; 5-Bromo-4-chloro-3-indolyl α-D-maltotriose; 3-Indolyl-α-D-maltotrioside, 5-bromo-4-chloro-; Magenta-α-D-maltotriose (common name); BCIP-α-Maltotriose; Chromogenic α-maltotriose substrate; α-Maltotriosyl-5-bromo-4-chloro-3-indolyl
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl-α-D-Maltotriose is manufactured under a strict quality management system. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Specific Rotation	Contact for details
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.