Description

.Alpha.-D-Glucofuranoside, 4-Nitrophenyl is a specialized glycoside derivative where a 4-nitrophenyl group is attached to the anomeric carbon of a D-glucofuranose ring. This compound is a valuable chromogenic substrate, releasing the intensely yellow 4-nitrophenolate ion upon enzymatic hydrolysis. It is primarily utilized as a critical reagent in biochemical research and industrial enzyme assays, particularly for measuring the activity of glycosidases like α-glucosidase. Industries involved in pharmaceutical development, diagnostic manufacturing, and life science research rely on this high-purity chemical for accurate and reproducible results.

Application

• Enzyme Substrate for Glycosidase Assays: Serves as a chromogenic substrate for the spectrophotometric determination of α-glucosidase and related enzyme activities in research and quality control.
• Biochemical Research: Used in kinetic studies, enzyme inhibition screening, and mechanistic investigations of carbohydrate-active enzymes.
• Diagnostic Kit Manufacturing: A key component in the formulation of clinical diagnostic kits for metabolic disorders, such as diabetes, where enzyme activity is a biomarker.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and characterize potential therapeutic inhibitors for metabolic diseases.
• Food & Beverage Analysis: Applied in enzymatic methods for the analysis of carbohydrate conversion and process monitoring in industrial biotechnology.
• Academic & Teaching Laboratories: Provides a reliable and standard reagent for educational experiments in enzymology and biochemistry.

Basic Information

Product Name	.Alpha.-D-Glucofuranoside, 4-Nitrophenyl
CAS No.	339094-38-1
Molecular Formula	C12H15NO8
Molecular Weight	301.25 g/mol
Synonyms	4-Nitrophenyl α-D-glucofuranoside; p-Nitrophenyl α-D-glucofuranoside; α-D-Glucofuranosyl 4-nitrobenzoate; PNPG (α-form); 4-Nitrophenyl-α-D-glucofuranoside; α-4-NPG; 1-O-(4-Nitrophenyl)-α-D-glucofuranose
EINECS	Contact for details

Quality Control

Every batch of .Alpha.-D-Glucofuranoside, 4-Nitrophenyl is manufactured and tested under a strict quality management system to ensure identity, purity, and performance consistency suitable for sensitive biochemical applications. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a dry environment to prevent absorption of water vapor, which can affect stability and assay performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.