Description

L-Aspartic Acid α-(7-Amido-4-Methylcoumarin), Trifluoroacetic Acid Salt is a high-purity, synthetic biochemical derivative that combines the amino acid L-aspartic acid with a fluorescent coumarin moiety. This compound is specifically engineered as a critical substrate and probe for enzymatic assays and biochemical research, offering high sensitivity and specificity. It is primarily required by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic research institutions focused on protease and peptidase activity studies.

Application

• Fluorogenic Substrate for Protease Assays: Used as a sensitive and selective substrate to measure the activity of aspartic proteases, caspases, and other specific peptidases in high-throughput screening (HTS).
• Biochemical Research Reagent: Serves as a key tool in enzymology studies to investigate enzyme kinetics, inhibition mechanisms, and substrate specificity.
• Diagnostic Development: Employed in the development of clinical diagnostic assays and kits for detecting enzymatic activity related to specific disease biomarkers.
• Pharmaceutical Drug Discovery: Integral to target validation and lead optimization processes in drug discovery programs focused on protease-targeted therapeutics.
• Cell Biology & Apoptosis Research: Utilized as a probe to monitor caspase activity and apoptotic pathways within cellular systems.

Basic Information

Item	Details
Product Name	L-Aspartic Acid α-(7-Amido-4-Methylcoumarin), Trifluoroacetic Acid Salt
CAS No.	336616-48-9
Molecular Formula	C₁₇H₁₇F₃N₂O₇
Molecular Weight	418.32 g/mol
Synonyms	Asp-AMC TFA Salt; L-Aspartic acid 7-amido-4-methylcoumarin amide TFA salt; Aspartic Acid AMC Derivative; AMC-Asp-OH•TFA; Caspase Substrate (Asp-AMC); Fluorogenic Peptide Substrate Asp-AMC; 7-Amido-4-methylcoumarin conjugate of L-Aspartic acid trifluoroacetate; Ac-Asp-AMC (related form)
EINECS	Contact for details

Quality Control

Every batch of our L-Aspartic Acid α-(7-Amido-4-Methylcoumarin), Trifluoroacetic Acid Salt is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and performance consistency. We adhere to stringent in-house specifications suitable for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, HPLC profile, and other critical parameters is provided with each shipment to guarantee traceability and quality assurance.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines
Fluorogenic Activity	Functional in standard assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.